Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling.

Chemiluminescence enzyme immunoassay enables to measure trace components with high sensitivity and selectivity due to high specificity of antigen-antibody reaction and high sensitivity chemiluminescence test. However, using enzyme-labeled antibodies suffer from problems such as low reproducibility due to the instability of the enzymes and the inhibition of antigen-antibody reaction due to its steric effect. Therefore, here we reported a chemiluminescence immunoassay innovative non-enzymatic labeling reagent through the use of quinone as a tag-signal generator coupled to biotin as a binder, to address the problem of enzymatic labeling.

Biotin-1,4-naphthoquinone (biotin-NQ) are synthesized and characterized and it can produce long lasting chemiluminescence upon mixing with dithiothreitol and luminol popularity quinone redox cycle. Biotin-NQ showed remarkable stability against stress factors of different organizations may face during an immunoassay such as high temperatures, conditions that are very acidic and alkaline, and repeated freeze-thaw cycles.

On the other hand, all of these conditions led to a decrease in the reactivity of the enzyme label because it may denature its protein structure. Finally, the biotin-labeled antibody measurements successfully performed using NQ-biotin and avidin. As a result, the antibody can be detected down to 25.7 nM were 2.5 times sensitive of biotin-HRP-enzyme immunoassay kemiluminesens. In addition, our method is successfully applied to the determination of the immobilized antibody using biotinylated avidin and biotin-NQ, which simulates immunoassay.

Avidin can be detected down to 23.4 nM with excellent linearity (r = 0.996). Accordingly, our advanced reagent, biotin-NQ, can be used as a very stable universal, cost-effective, and steric free non-enzymatic labels for immunoassay.

DMP-543

T15143-5mg 5mg Ask for price
Description: DMP-543

DMP-543

MBS3844443-10mg 10mg
EUR 1905

DMP-543

MBS3844443-1mg 1mg
EUR 430

DMP-543

MBS3844443-5mg 5mg
EUR 1165

DMP-543

MBS3844443-5x10mg 5x10mg
EUR 8565

DMP-777

MBS3602248-25mg 25mg
EUR 540

DMP-777

MBS3602248-50mg 50mg
EUR 770

DMP-777

MBS3602248-5x50mg 5x50mg
EUR 3150

DMP-543

MBS5768992-200mg 200mg
EUR 1315

DMP-543

MBS5768992-5x200mg 5x200mg
EUR 5775
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