Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling.

Chemiluminescence enzyme immunoassay enables to measure trace components with high sensitivity and selectivity due to high specificity of antigen-antibody reaction and high sensitivity chemiluminescence test. However, using enzyme-labeled antibodies suffer from problems such as low reproducibility due to the instability of the enzymes and the inhibition of antigen-antibody reaction due to its steric effect. Therefore, here we reported a chemiluminescence immunoassay innovative non-enzymatic labeling reagent through the use of quinone as a tag-signal generator coupled to biotin as a binder, to address the problem of enzymatic labeling.

Biotin-1,4-naphthoquinone (biotin-NQ) are synthesized and characterized and it can produce long lasting chemiluminescence upon mixing with dithiothreitol and luminol popularity quinone redox cycle. Biotin-NQ showed remarkable stability against stress factors of different organizations may face during an immunoassay such as high temperatures, conditions that are very acidic and alkaline, and repeated freeze-thaw cycles.

On the other hand, all of these conditions led to a decrease in the reactivity of the enzyme label because it may denature its protein structure. Finally, the biotin-labeled antibody measurements successfully performed using NQ-biotin and avidin. As a result, the antibody can be detected down to 25.7 nM were 2.5 times sensitive of biotin-HRP-enzyme immunoassay kemiluminesens. In addition, our method is successfully applied to the determination of the immobilized antibody using biotinylated avidin and biotin-NQ, which simulates immunoassay.

Avidin can be detected down to 23.4 nM with excellent linearity (r = 0.996). Accordingly, our advanced reagent, biotin-NQ, can be used as a very stable universal, cost-effective, and steric free non-enzymatic labels for immunoassay.

ELISA kit for Mouse DMP-1
EK5579 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse DMP-1 in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse DMP-1 PicoKine ELISA Kit
EK1290 96 wells
EUR 510
Description: For quantitative detection of mouse DMP-1 in cell culture supernates, serum and plasma(heparin, EDTA).

DMP-1 ELISA Kit (Mouse) (OKBB00584)
OKBB00584 96 Wells
EUR 606
Description: Description of target: Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Human dimethyl phthalate (DMP)
EHD0277 96Tests
EUR 625.2

Fmoc-Ala-Cys(Psi(Dmp,H)pro)-OH
B-4385.0001 1.0g
EUR 290.4
Description: Sum Formula: C30H30N2O7S; CAS# [2022956-37-0]

Fmoc-Gly-Cys(Psi(Dmp,H)pro)-OH
B-4395.0001 1.0g
EUR 304.8
Description: Sum Formula: C29H28N2O7S; CAS# [1926163-05-4]

Fmoc-Leu-Cys(Psi(Dmp,H)pro)-OH
B-4400.0001 1.0g
EUR 304.8
Description: Sum Formula: C33H36N2O7S; CAS# [1926163-06-5]

Fmoc-Val-Cys(Psi(Dmp,H)pro)-OH
B-4410.0001 1.0g
EUR 304.8
Description: Sum Formula: C32H34N2O7S; CAS# [1926163-08-7]

Fmoc-Cys(Trt)-Cys(Psi(Dmp,H)pro)-OH
B-4390.0250 250.0mg
EUR 432
Description: Sum Formula: C49H44N2O7S2; CAS# [2022956-75-6]

Fmoc-Lys(Boc)-Cys(Psi(Dmp,H)pro)-OH
B-4405.0001 1.0g
EUR 304.8
Description: Sum Formula: C38H45N3O9S; CAS# [1926163-07-6]
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