Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications.

Antibodies are widely used as a cancer therapy, but their use is currently limited by the low number of limited antigen cancer cells. A receptor tyrosine kinase, receptor tyrosine kinase-like orphan receptor 2 (ROR2), typically expressed only during embryogenesis and close down-regulated in healthy tissues after birth, but up-regulated in a diverse set of hematological and solid malignancies.

Thus, ROR2 a candidate antigen for antibody-based cancer therapy. Here we describe the affinity maturation and humanization of a rabbit mAb that binds human and rat ROR2 but ROR1 no human or human cells other surface antigens. Co-crystallization of the rabbit mAb complexes with human parents ROR2 kringle domains (hROR2-Kr) guided affinity maturation by a heavy chain complementarity-determination of the region 3 (HCDR3) -focused mutagenesis and selection.

Affinity-matured rabbit mAb then humanized by a complementary-determination region (CDR) grafting and framework fine tuning, and more co-crystallized with hROR2-Kr. We show that the affinity matured and humanized mAb retains a strong affinity and specificity to ROR2 and, after conversion to the T cell engaging bispecific antibody, has potent cytotoxicity towards ROR2-expressing cells. We anticipate that this human affinity matured mAb will find applications for antibody-based cancer therapy of ROR2-expressing neoplasms.

TBS, pH7.6 - Buffer for Dot Blot and Western Blot Kits

WB-B002 50 ml
EUR 18.36

Western Blot Blocker

20960007-1 250 g
EUR 32.46

Western Blot Blocker

20960007-2 500 g
EUR 60.54

TBST, pH7.6 - Buffer for Dot Blot and Western Blot Kits

WB-B001 100 ml
EUR 25.92

Western Blot Kit

20-abx098123
  • Ask for price
  • Ask for price
  • 100 ml
  • 200 ml

Western Blot Kit

abx098123-100l 100 Āµl
EUR 325

Western Blot Kit

abx098123-1ml 1 ml Ask for price

Western Blot Kit

abx098123-200l 200 Āµl
EUR 400

Detection Reagent A for Dot Blot and Western Blot Kits

WB-B006 30 ml
EUR 86.4

Detection Reagent B for Dot Blot and Western Blot Kits

WB-B007 30 ml
EUR 86.4

Western Blot Marker

TTOPP-2301 20Lanes
EUR 310.44

Bovine DNAse 1 Protein Western blot +ve control

DNASE11-C 100 ul
EUR 343.2

ID4 Western Blot Kit

GWB-232633 Kit Ask for price

ERG Western Blot Kit

GWB-589385 Kit Ask for price

SSB Western Blot Kit

GWB-4759BD Kit Ask for price

LARGE BLOT BOX - CLEAR

BLWVS0029 11.7x9.0x2.9CM
EUR 45.36

FST Western Blot Kit

GWB-31B3A5 Kit Ask for price

EED Western Blot Kit

GWB-31EF96 Kit Ask for price

AHR Western Blot Kit

GWB-EFD465 Kit Ask for price

OTC Western Blot Kit

GWB-CED6C6 Kit Ask for price

ENG Western Blot Kit

GWB-C2F17D Kit Ask for price

MSI2 Western Blot Kit

GWB-24AE67 Kit Ask for price

GLI2 Western Blot Kit

GWB-2A323E Kit Ask for price

TSHR Western Blot Kit

GWB-2D5460 Kit Ask for price

BRD7 Western Blot Kit

GWB-51BDA0 Kit Ask for price

ILF2 Western Blot Kit

GWB-1149E0 Kit Ask for price

LHX6 Western Blot Kit

GWB-13F4C0 Kit Ask for price

ASPN Western Blot Kit

GWB-3F3BF8 Kit Ask for price

SPP1 Western Blot Kit

GWB-4172CB Kit Ask for price

ETS1 Western Blot Kit

GWB-419F49 Kit Ask for price

G3BP Western Blot Kit

GWB-46B393 Kit Ask for price

TPM1 Western Blot Kit

GWB-16A472 Kit Ask for price

SMALL BLOT BOX - YELLOW

ACCBW0010 7.3x5.1x3.3CM
EUR 37.95

LARGE BLOT BOX - YELLOW

ACCBW0012 11.7x9.0x2.9CM
EUR 53.26

SMALL BLOT BOX - ORANGE

ACCBW0015 7.3x5.1x3.3CM
EUR 37.95

LARGE BLOT BOX - ORANGE

ACCBW0017 11.7x9.0x2.9CM
EUR 53.26

MEDIUM BLOT BOX - CLEAR

BLWVS0028 9.1x6.6x2.9CM
EUR 32.42

NPM1 Western Blot Kit

GWB-AA50AA Kit Ask for price

RNF2 Western Blot Kit

GWB-31E776 Kit Ask for price

ZBP1 Western Blot Kit

GWB-5E94FB Kit Ask for price

RORA Western Blot Kit

GWB-DC713B Kit Ask for price

PAX7 Western Blot Kit

GWB-DF90D0 Kit Ask for price

PAX7 Western Blot Kit

GWB-EEA80D Kit Ask for price

GLI1 Western Blot Kit

GWB-888E77 Kit Ask for price

SOX5 Western Blot Kit

GWB-908D84 Kit Ask for price

A1BG Western Blot Kit

GWB-FA7CD7 Kit Ask for price

LIG4 Western Blot Kit

GWB-FAE800 Kit Ask for price

STAU1 Western Blot Kit

GWB-236BD7 Kit Ask for price

GABRP Western Blot Kit

GWB-24C55E Kit Ask for price

TRPM3 Western Blot Kit

GWB-28C4BA Kit Ask for price

HIPK2 Western Blot Kit

GWB-2CAB70 Kit Ask for price

ESRRG Western Blot Kit

GWB-306860 Kit Ask for price

ANXA1 Western Blot Kit

GWB-11A960 Kit Ask for price

SATB1 Western Blot Kit

GWB-1505B2 Kit Ask for price

RSAD2 Western Blot Kit

GWB-158EF5 Kit Ask for price

PDCD4 Western Blot Kit

GWB-37F683 Kit Ask for price

TNRC4 Western Blot Kit

GWB-3AECE0 Kit Ask for price

GNB1L Western Blot Kit

GWB-3C89D6 Kit Ask for price

PITX2 Western Blot Kit

GWB-3D38F8 Kit Ask for price

RPS16 Western Blot Kit

GWB-407949 Kit Ask for price

STK11 Western Blot Kit

GWB-4A089E Kit Ask for price

PTPN2 Western Blot Kit

GWB-4A1E76 Kit Ask for price

MEDIUM BLOT BOX - YELLOW

ACCBW0011 9.1x6.6x2.9CM
EUR 41.55

MEDIUM BLOT BOX - ORANGE

ACCBW0016 9.1x6.6x2.9CM
EUR 41.55

ZFP36 Western Blot Kit

GWB-B1BCFA Kit Ask for price

ZFP67 Western Blot Kit

GWB-9EC907 Kit Ask for price

WNT2B Western Blot Kit

GWB-31DC35 Kit Ask for price

IDH3A Western Blot Kit

GWB-5ED2E6 Kit Ask for price

PCBP1 Western Blot Kit

GWB-7D2B23 Kit Ask for price

CLIC1 Western Blot Kit

GWB-DA38C2 Kit Ask for price

ARIH2 Western Blot Kit

GWB-DB4558 Kit Ask for price

PBEF1 Western Blot Kit

GWB-EA0DE7 Kit Ask for price

RAB14 Western Blot Kit

GWB-EF804C Kit Ask for price

HTR1A Western Blot Kit

GWB-843597 Kit Ask for price

P2RX7 Western Blot Kit

GWB-8C5BF1 Kit Ask for price

KCNN2 Western Blot Kit

GWB-8E971E Kit Ask for price

SOX10 Western Blot Kit

GWB-93F38B Kit Ask for price

OLIG2 Western Blot Kit

GWB-6EC267 Kit Ask for price

PANX2 Western Blot Kit

GWB-F284D9 Kit Ask for price

CLCN3 Western Blot Kit

GWB-F33E4A Kit Ask for price

TGFBI Western Blot Kit

GWB-F84989 Kit Ask for price

PANX1 Western Blot Kit

GWB-FD36EC Kit Ask for price

RUNX2 Western Blot Kit

GWB-FFA7E7 Kit Ask for price

ZFP42 Western Blot Kit

GWB-CABDE0 Kit Ask for price

KEAP1 Western Blot Kit

GWB-CD9C1D Kit Ask for price

ATP7A Western Blot Kit

GWB-D12842 Kit Ask for price

KRT13 Western Blot Kit

GWB-C3423B Kit Ask for price

JMJD3 Western Blot Kit

GWB-D8EFB3 Kit Ask for price

HNRPA3 Western Blot Kit

GWB-1EAFD5 Kit Ask for price

SNRP70 Western Blot Kit

GWB-12C365 Kit Ask for price

TARDBP Western Blot Kit

GWB-3E00CF Kit Ask for price

TWIST1 Western Blot Kit

GWB-172C7E Kit Ask for price

TARDBP Western Blot Kit

GWB-DC48CE Kit Ask for price

HNRPLL Western Blot Kit

GWB-72079F Kit Ask for price

SLC1A5 Western Blot Kit

GWB-F244A5 Kit Ask for price

Blocking Buffer and Antibody Diluent for Dot Blot and Western Blot Kits

WB-B003 50 ml
EUR 66.96

RHOBTB1 Western Blot Kit

GWB-16F5AC Kit Ask for price

SLC22A7 Western Blot Kit

GWB-A3D4FB Kit Ask for price

CEACAM6 Western Blot Kit

GWB-DA1310 Kit Ask for price

ONECUT2 Western Blot Kit

GWB-D5E58E Kit Ask for price

SLC22A1 Western Blot Kit

GWB-D6EAC0 Kit Ask for price

SLC12A1 Western Blot Kit

GWB-F10DDC Kit Ask for price

THICK BLOT PAPER - 10X10CM

IB95011 10 SHEETS - 0.8MM THCK
EUR 49.99

Rat Placenta Western Blot

RW-413 1 Blot
EUR 668

Western Blot Enhancing Kit

K3172120 1200 cm2
EUR 123

Western Blot Enhancing Kit

K3172250 2500 cm2
EUR 182

Western Blot Detection Kit

E-IR-R304A-50Assays 50Assays
EUR 210

Western Blot Detection Kit

E-IR-R304A-each each Ask for price

WSE-4055 Q Blot kit

2322445 2unit
EUR 462

Human Cell Line II Blot

1502 One blot
EUR 544.2
Description: The human cell line blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human cell lines with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The cell lines included in the blot are Daudi (B lymphoblast; Burkkit's lymphoma), Raji (B lymphoblast; Burkkit's lymphoma), K-562 (bone marrow; chronic myelogenous leukmia), U-937 (histiocytic lymphoma), THP-1 (monocyte; acute monocytic leukmia), HL-60 (promyeloblast; acute promyeloblastic leukmia), Jurkat (T lymphocyte; acute T cell leukmia), and MOLT-4 (T lymphoblast; acute lymphoblastic leukmia).

LL37 (Human) - Dot Blot Kit

DBK-075-06 5 blots
EUR 805.68

Human Lung Tumor Blot VI

1546 One blot
EUR 802.5
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 1 normal human lung tissue, the cell line A549 (human lung carcinoma), and 7 different human lung tumor tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins in a wide range of molecular mass are separated in a 4-20% gradient SDS-PAGE gel and transferred onto nitrocellulose membranes. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, 1 differentiated adenocarcinoma, 1 squamous cell carcinoma, 1 pleomorphic carcinoma and 2 non-diagnosed carcinomas (from different donors).

X-LARGE BLOT BOX - CLEAR

ACCBW0004 15.4x10.4x3.6CM
EUR 57.73

Rat Normal Tissue Blot I

1581 One blot
EUR 493.8
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The rat tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Rat Normal Tissue Blot II

1582 One blot
EUR 493.8
Description: The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.

mini blot insert 10x10 cm

EVS1100-BLOTINSERT ea
EUR 493.2

maxi blot insert 20x20 cm

EVS1300-BLOTINSERT ea
EUR 706.8

X-LARGE BLOT BOX - YELLOW

ACCBW0013 15.4x10.4x3.6CM
EUR 65.12

X-LARGE BLOT BOX - ORANGE

ACCBW0018 15.4x10.4x3.6CM
EUR 63.91

Human Tumor Tissue Blot I

1542 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human tumor tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human tumor tissues included in the blot are brain, colon, kidney, liver, lung, pancreas, skin, spleen, and stomach.

Human Tumor Tissue Blot II

1543 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human tumor tissues included in the blot are colon, duodenum, esophagus, small intestine, liver, pancreas, rectum, and stomach.

Human Tumor Tissue Blot IV

1545 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human tumor tissues included in the blot are kidney, liver, lymphoma, Non-Hodgkin's lymphoma, spleen, thymoma, thyroid, and tonsil.

mini blot casette 10x10 cm

EVS1100-CASETTE ea
EUR 78

maxi blot casette 20x20 cm

EVS1300-CASETTE ea
EUR 98.4

mini blot casette 10x10 cm

EVS3100-CASETTE ea
EUR 158.4

maxi blot casette 20x20 cm

EVS3300-CASETTE ea
EUR 189.6

cyclin G2 Western Blot Kit

GWB-127000 Kit Ask for price

Human Normal Tissue Blot II

1522 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot VI

1526 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

Human Tumor Tissue Blot III

1544 One blot
EUR 638.7
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human tumor tissues included in the blot are bladder, breast, kidney, ovary, prostate, testis, cervix, and uterus.

Mouse Normal Tissue Blot I

1561 One blot
EUR 493.8
Description: The mouse tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different mouse tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The mouse tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Mouse Normal Tissue Blot II

1562 One blot
EUR 493.8
Description: The mouse tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different mouse tissue lysates with 15 Āµg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The mouse tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.

Sub Blot Mini Cassette - EACH

SB10C EACH
EUR 68.85

Sub Blot Maxi Cassette - EACH

SB20C EACH
EUR 114.75

Skin Western Blot, 14 species

AW-101 1 Blot
EUR 1031

Lung Western Blot, 14 species

AW-601 1 Blot
EUR 1031

miniwide blot insert 20x10 cm

EVS1200-BLOTINSERT ea
EUR 564

Brain Western Blot, 14 species

AW-201 1 Blot
EUR 1031

Colon Western Blot, 14 species

AW-311 1 Blot
EUR 1031

Liver Western Blot, 14 species

AW-314 1 Blot
EUR 1031

Ovary Western Blot, 14 species

AW-406 1 Blot
EUR 1031

Heart Western Blot, 14 species

AW-801 1 Blot
EUR 1031

Rat Whole Embryo Western Blot

RW-104 1 Blot
EUR 668

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 992.4
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 298.8
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

Testis Western Blot, 14 species

AW-401 1 Blot
EUR 1031

Kidney Western Blot, 14 species

AW-901 1 Blot
EUR 1031

AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Rat Mammary Gland Western Blot

RW-414 1 Blot
EUR 668

Augurin (Human) - Western Blot Kit

WBK-012-25 1 kit
EUR 822.96

Ghrelin (Human) - Western Blot Kit

WBK-031-30 1 kit
EUR 822.96

Dog Tissue Premade Western Blot

DW-MT-1 1 Blot
EUR 1031

Muscles Western Blot, 14 species

AW-102 1 Blot
EUR 1031

Stomach Western Blot, 14 species

AW-302 1 Blot
EUR 1031

ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity: Human
  • Application:
  • Assay info:
  • Sensitivity:

NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 776.4
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:
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