Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications.

Antibodies are widely used as a cancer therapy, but their use is currently limited by the low number of limited antigen cancer cells. A receptor tyrosine kinase, receptor tyrosine kinase-like orphan receptor 2 (ROR2), typically expressed only during embryogenesis and close down-regulated in healthy tissues after birth, but up-regulated in a diverse set of hematological and solid malignancies.

Thus, ROR2 a candidate antigen for antibody-based cancer therapy. Here we describe the affinity maturation and humanization of a rabbit mAb that binds human and rat ROR2 but ROR1 no human or human cells other surface antigens. Co-crystallization of the rabbit mAb complexes with human parents ROR2 kringle domains (hROR2-Kr) guided affinity maturation by a heavy chain complementarity-determination of the region 3 (HCDR3) -focused mutagenesis and selection.

Affinity-matured rabbit mAb then humanized by a complementary-determination region (CDR) grafting and framework fine tuning, and more co-crystallized with hROR2-Kr. We show that the affinity matured and humanized mAb retains a strong affinity and specificity to ROR2 and, after conversion to the T cell engaging bispecific antibody, has potent cytotoxicity towards ROR2-expressing cells. We anticipate that this human affinity matured mAb will find applications for antibody-based cancer therapy of ROR2-expressing neoplasms.

Scl-70, Recombinant, Human, Western Blot Positive Control (Scleroderma, DNA Topoisomerase I)
MBS637451-01mL 0.1mL
EUR 575

Scl-70, Recombinant, Human, Western Blot Positive Control (Scleroderma, DNA Topoisomerase I)
MBS637451-5x01mL 5x0.1mL
EUR 2440

BlotBot Mini Blot Tray
TRAY-2MINI-2 2pack
EUR 244.8
Description: Replacement trays for the BlotBot allows you to run 2 Mini sized blots simultaneaously. Pack of 2 trays.

BlotBot Midi Blot Tray
TRAY-MIDI-2 2pack
EUR 242.4
Description: Replacement tray for the BlotBot allows you to run 1 Midi sized blot. Pack of 2 trays.

TBS, pH7.6 - Buffer for Dot Blot and Western Blot Kits
WB-B002 50 ml
EUR 18.36

BLOT WASHER - 115V
BLWAA115S -
EUR 2585.32

BLOT WASHER - 220V
BLWAA220S -
EUR 2585.32

TBST, pH7.6 - Buffer for Dot Blot and Western Blot Kits
WB-B001 100 ml
EUR 25.92

Western Blot Blocker
20960007-1 250 g
EUR 39.81

Western Blot Blocker
20960007-2 500 g
EUR 74.25

Western Blot Kit
abx098123-100l 100 µl
EUR 325

Western Blot Kit
20-abx098123
  • Ask for price
  • Ask for price
  • 100 ml
  • 200 ml

Western Blot Kit
abx098123-1ml 1 ml Ask for price

Western Blot Kit
abx098123-200l 200 µl
EUR 400

Detection Reagent A for Dot Blot and Western Blot Kits
WB-B006 30 ml
EUR 86.4

Detection Reagent B for Dot Blot and Western Blot Kits
WB-B007 30 ml
EUR 86.4

Western Blot Marker
TTOPP-2301 20Lanes
EUR 310.44

Western Blot Reagent
E45O49346O9 5 ml
EUR 1191.67

Western Blot Reagent
E45O49347O9 1 ml
EUR 316.88

Western Blot Reagent
E45O49348O9 5 ml
EUR 1270.21

Western Blot Reagent
E45O49349O9 1 ml
EUR 343.96

LARGE BLOT BOX - CLEAR
BLWVS0029 11.7x9.0x2.9CM
EUR 45.36

SSB Western Blot Kit
GWB-4759BD Kit Ask for price

ERG Western Blot Kit
GWB-589385 Kit Ask for price

ID4 Western Blot Kit
GWB-232633 Kit Ask for price

FST Western Blot Kit
GWB-31B3A5 Kit Ask for price

EED Western Blot Kit
GWB-31EF96 Kit Ask for price

AHR Western Blot Kit
GWB-EFD465 Kit Ask for price

ENG Western Blot Kit
GWB-C2F17D Kit Ask for price

OTC Western Blot Kit
GWB-CED6C6 Kit Ask for price

ERG Western Blot kit
MBS3224720-10Reactions 10Reactions
EUR 675

ERG Western Blot kit
MBS3224720-5x10Reactions 5x10Reactions
EUR 2850

AHR Western Blot kit
MBS3224728-10Reactions 10Reactions
EUR 675

AHR Western Blot kit
MBS3224728-5x10Reactions 5x10Reactions
EUR 2850

ENG Western Blot kit
MBS3224736-10Reactions 10Reactions
EUR 675

ENG Western Blot kit
MBS3224736-5x10Reactions 5x10Reactions
EUR 2850

ID4 Western Blot kit
MBS3224764-10Reactions 10Reactions
EUR 675

ID4 Western Blot kit
MBS3224764-5x10Reactions 5x10Reactions
EUR 2850

EED Western Blot kit
MBS3224766-10Reactions 10Reactions
EUR 675

EED Western Blot kit
MBS3224766-5x10Reactions 5x10Reactions
EUR 2850

SSB Western Blot kit
MBS3224772-10Reactions 10Reactions
EUR 675

SSB Western Blot kit
MBS3224772-5x10Reactions 5x10Reactions
EUR 2850

FST Western Blot kit
MBS3224792-10Reactions 10Reactions
EUR 675

FST Western Blot kit
MBS3224792-5x10Reactions 5x10Reactions
EUR 2850

SMALL BLOT BOX - YELLOW
ACCBW0010 7.3x5.1x3.3CM
EUR 37.95

LARGE BLOT BOX - YELLOW
ACCBW0012 11.7x9.0x2.9CM
EUR 53.26

SMALL BLOT BOX - ORANGE
ACCBW0015 7.3x5.1x3.3CM
EUR 37.95

LARGE BLOT BOX - ORANGE
ACCBW0017 11.7x9.0x2.9CM
EUR 53.26

MEDIUM BLOT BOX - CLEAR
BLWVS0028 9.1x6.6x2.9CM
EUR 32.42

G3BP Western Blot Kit
GWB-46B393 Kit Ask for price

BRD7 Western Blot Kit
GWB-51BDA0 Kit Ask for price

ZBP1 Western Blot Kit
GWB-5E94FB Kit Ask for price

NPM1 Western Blot Kit
GWB-AA50AA Kit Ask for price

MSI2 Western Blot Kit
GWB-24AE67 Kit Ask for price

GLI2 Western Blot Kit
GWB-2A323E Kit Ask for price

GLI1 Western Blot Kit
GWB-888E77 Kit Ask for price

SOX5 Western Blot Kit
GWB-908D84 Kit Ask for price

TSHR Western Blot Kit
GWB-2D5460 Kit Ask for price

RNF2 Western Blot Kit
GWB-31E776 Kit Ask for price

ASPN Western Blot Kit
GWB-3F3BF8 Kit Ask for price

SPP1 Western Blot Kit
GWB-4172CB Kit Ask for price

ETS1 Western Blot Kit
GWB-419F49 Kit Ask for price

ILF2 Western Blot Kit
GWB-1149E0 Kit Ask for price

LHX6 Western Blot Kit
GWB-13F4C0 Kit Ask for price

TPM1 Western Blot Kit
GWB-16A472 Kit Ask for price

RORA Western Blot Kit
GWB-DC713B Kit Ask for price

PAX7 Western Blot Kit
GWB-DF90D0 Kit Ask for price

A1BG Western Blot Kit
GWB-FA7CD7 Kit Ask for price

LIG4 Western Blot Kit
GWB-FAE800 Kit Ask for price

PAX7 Western Blot Kit
GWB-EEA80D Kit Ask for price

ETS1 Western Blot kit
MBS3224719-10Reactions 10Reactions
EUR 675

ETS1 Western Blot kit
MBS3224719-5x10Reactions 5x10Reactions
EUR 2850

GLI2 Western Blot kit
MBS3224726-10Reactions 10Reactions
EUR 675

GLI2 Western Blot kit
MBS3224726-5x10Reactions 5x10Reactions
EUR 2850

PAX4 Western Blot kit
MBS3224727-10Reactions 10Reactions
EUR 675

PAX4 Western Blot kit
MBS3224727-5x10Reactions 5x10Reactions
EUR 2850

GLI1 Western Blot kit
MBS3224729-10Reactions 10Reactions
EUR 675

GLI1 Western Blot kit
MBS3224729-5x10Reactions 5x10Reactions
EUR 2850

PAX7 Western Blot kit
MBS3224730-10Reactions 10Reactions
EUR 675

PAX7 Western Blot kit
MBS3224730-5x10Reactions 5x10Reactions
EUR 2850

LHX6 Western Blot kit
MBS3224731-10Reactions 10Reactions
EUR 675

LHX6 Western Blot kit
MBS3224731-5x10Reactions 5x10Reactions
EUR 2850

PAX7 Western Blot kit
MBS3224733-10Reactions 10Reactions
EUR 675

PAX7 Western Blot kit
MBS3224733-5x10Reactions 5x10Reactions
EUR 2850

SOX5 Western Blot kit
MBS3224737-10Reactions 10Reactions
EUR 675

SOX5 Western Blot kit
MBS3224737-5x10Reactions 5x10Reactions
EUR 2850

A1BG Western Blot kit
MBS3224741-10Reactions 10Reactions
EUR 675

A1BG Western Blot kit
MBS3224741-5x10Reactions 5x10Reactions
EUR 2850

LIG4 Western Blot kit
MBS3224743-10Reactions 10Reactions
EUR 675

LIG4 Western Blot kit
MBS3224743-5x10Reactions 5x10Reactions
EUR 2850

RNF2 Western Blot kit
MBS3224744-10Reactions 10Reactions
EUR 675

RNF2 Western Blot kit
MBS3224744-5x10Reactions 5x10Reactions
EUR 2850

ILF2 Western Blot kit
MBS3224754-10Reactions 10Reactions
EUR 675

ILF2 Western Blot kit
MBS3224754-5x10Reactions 5x10Reactions
EUR 2850

SPP1 Western Blot kit
MBS3224758-10Reactions 10Reactions
EUR 675

SPP1 Western Blot kit
MBS3224758-5x10Reactions 5x10Reactions
EUR 2850

ZBP1 Western Blot kit
MBS3224760-10Reactions 10Reactions
EUR 675

ZBP1 Western Blot kit
MBS3224760-5x10Reactions 5x10Reactions
EUR 2850

G3BP Western Blot kit
MBS3224762-10Reactions 10Reactions
EUR 675

G3BP Western Blot kit
MBS3224762-5x10Reactions 5x10Reactions
EUR 2850

BRD7 Western Blot kit
MBS3224769-10Reactions 10Reactions
EUR 675

BRD7 Western Blot kit
MBS3224769-5x10Reactions 5x10Reactions
EUR 2850

MSI2 Western Blot kit
MBS3224776-10Reactions 10Reactions
EUR 675

MSI2 Western Blot kit
MBS3224776-5x10Reactions 5x10Reactions
EUR 2850

TPM1 Western Blot kit
MBS3224781-10Reactions 10Reactions
EUR 675

TPM1 Western Blot kit
MBS3224781-5x10Reactions 5x10Reactions
EUR 2850

TSHR Western Blot kit
MBS3224786-10Reactions 10Reactions
EUR 675

TSHR Western Blot kit
MBS3224786-5x10Reactions 5x10Reactions
EUR 2850

ASPN Western Blot kit
MBS3224794-10Reactions 10Reactions
EUR 675

ASPN Western Blot kit
MBS3224794-5x10Reactions 5x10Reactions
EUR 2850

RORA Western Blot kit
MBS3224800-10Reactions 10Reactions
EUR 675

RORA Western Blot kit
MBS3224800-5x10Reactions 5x10Reactions
EUR 2850

MEDIUM BLOT BOX - YELLOW
ACCBW0011 9.1x6.6x2.9CM
EUR 41.55

MEDIUM BLOT BOX - ORANGE
ACCBW0016 9.1x6.6x2.9CM
EUR 41.55

STK11 Western Blot Kit
GWB-4A089E Kit Ask for price

PTPN2 Western Blot Kit
GWB-4A1E76 Kit Ask for price

IDH3A Western Blot Kit
GWB-5ED2E6 Kit Ask for price

ZFP67 Western Blot Kit
GWB-9EC907 Kit Ask for price

ZFP36 Western Blot Kit
GWB-B1BCFA Kit Ask for price

STAU1 Western Blot Kit
GWB-236BD7 Kit Ask for price

GABRP Western Blot Kit
GWB-24C55E Kit Ask for price

TRPM3 Western Blot Kit
GWB-28C4BA Kit Ask for price

OLIG2 Western Blot Kit
GWB-6EC267 Kit Ask for price

PCBP1 Western Blot Kit
GWB-7D2B23 Kit Ask for price

P2RX7 Western Blot Kit
GWB-8C5BF1 Kit Ask for price

KCNN2 Western Blot Kit
GWB-8E971E Kit Ask for price

SOX10 Western Blot Kit
GWB-93F38B Kit Ask for price

PDCD4 Western Blot Kit
GWB-37F683 Kit Ask for price

TNRC4 Western Blot Kit
GWB-3AECE0 Kit Ask for price

GNB1L Western Blot Kit
GWB-3C89D6 Kit Ask for price

PITX2 Western Blot Kit
GWB-3D38F8 Kit Ask for price

HIPK2 Western Blot Kit
GWB-2CAB70 Kit Ask for price

ESRRG Western Blot Kit
GWB-306860 Kit Ask for price

WNT2B Western Blot Kit
GWB-31DC35 Kit Ask for price

RPS16 Western Blot Kit
GWB-407949 Kit Ask for price

HTR1A Western Blot Kit
GWB-843597 Kit Ask for price

ANXA1 Western Blot Kit
GWB-11A960 Kit Ask for price

SATB1 Western Blot Kit
GWB-1505B2 Kit Ask for price

RSAD2 Western Blot Kit
GWB-158EF5 Kit Ask for price

CLIC1 Western Blot Kit
GWB-DA38C2 Kit Ask for price

ARIH2 Western Blot Kit
GWB-DB4558 Kit Ask for price

PBEF1 Western Blot Kit
GWB-EA0DE7 Kit Ask for price

PANX2 Western Blot Kit
GWB-F284D9 Kit Ask for price

CLCN3 Western Blot Kit
GWB-F33E4A Kit Ask for price

TGFBI Western Blot Kit
GWB-F84989 Kit Ask for price

PANX1 Western Blot Kit
GWB-FD36EC Kit Ask for price

RUNX2 Western Blot Kit
GWB-FFA7E7 Kit Ask for price

JMJD3 Western Blot Kit
GWB-D8EFB3 Kit Ask for price

ATP7A Western Blot Kit
GWB-D12842 Kit Ask for price

RAB14 Western Blot Kit
GWB-EF804C Kit Ask for price

KRT13 Western Blot Kit
GWB-C3423B Kit Ask for price

ZFP42 Western Blot Kit
GWB-CABDE0 Kit Ask for price

KEAP1 Western Blot Kit
GWB-CD9C1D Kit Ask for price

PDCD4 Western Blot kit
MBS3224718-10Reactions 10Reactions
EUR 675

PDCD4 Western Blot kit
MBS3224718-5x10Reactions 5x10Reactions
EUR 2850

GABRP Western Blot kit
MBS3224721-10Reactions 10Reactions
EUR 675

GABRP Western Blot kit
MBS3224721-5x10Reactions 5x10Reactions
EUR 2850

HTR1A Western Blot kit
MBS3224722-10Reactions 10Reactions
EUR 675

HTR1A Western Blot kit
MBS3224722-5x10Reactions 5x10Reactions
EUR 2850

RAB14 Western Blot kit
MBS3224723-10Reactions 10Reactions
EUR 675

RAB14 Western Blot kit
MBS3224723-5x10Reactions 5x10Reactions
EUR 2850

TNRC4 Western Blot kit
MBS3224724-10Reactions 10Reactions
EUR 675

TNRC4 Western Blot kit
MBS3224724-5x10Reactions 5x10Reactions
EUR 2850

ESRRG Western Blot kit
MBS3224725-10Reactions 10Reactions
EUR 675

ESRRG Western Blot kit
MBS3224725-5x10Reactions 5x10Reactions
EUR 2850

HIPK2 Western Blot kit
MBS3224732-10Reactions 10Reactions
EUR 675

HIPK2 Western Blot kit
MBS3224732-5x10Reactions 5x10Reactions
EUR 2850

ZFP67 Western Blot kit
MBS3224734-10Reactions 10Reactions
EUR 675

ZFP67 Western Blot kit
MBS3224734-5x10Reactions 5x10Reactions
EUR 2850

OLIG2 Western Blot kit
MBS3224735-10Reactions 10Reactions
EUR 675

OLIG2 Western Blot kit
MBS3224735-5x10Reactions 5x10Reactions
EUR 2850

SOX10 Western Blot kit
MBS3224738-10Reactions 10Reactions
EUR 675
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