Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.

Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.

Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.
Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.

ECL Western Blotting Substrate

KF005 100ml
EUR 140

Blotting Paper, 10X15cm, 100 Sheets

PP3322 1PK, 100UNIT
EUR 105.68

Blotting Paper, 15X15cm, 100 Sheets

PP3323 1PK, 100UNIT
EUR 117.04

Blotting Paper, 20X20cm, 100 Sheets

PP3324 1PK, 100UNIT
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ECL Plus Western Blotting Substrate

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EUR 101

ECL Western Blotting Substrate Kit

K2187-50 50 assays
EUR 160

ECL Western Blotting Substrate Kit

K2187-500 500 assays
EUR 363

WESTSAVE Up (Western Blotting Substrate)

LF-QC0101 1 kit
EUR 151
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

ECL Western Blotting Substrate Kit

42R-1001 500 ml
EUR 426
Description: ECL Western Blotting Substrate Kit for use in the research laboratory

ECL Western Blotting Substrate Kit

K820-50
EUR 153

ECL Western Blotting Substrate Kit

K820-500
EUR 338

T-Pro Transfer Blotting buffer (10X)

JB08-H001 500ml/BT
EUR 135

WESTSAVE Up (Western Blotting Substrate, double pack)

LF-QC0102 1 kit(Double pack)
EUR 222
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

Western Blotting Filter Paper, 12.5 cm—12.5 cm

AR0172 100 sheets
EUR 70

Western Blotting Filter Paper, 9 cm— 7.5 cm

AR0173 100 sheets
EUR 65

Purified Nipah virus Glycoprotein control for Western Blotting

NIV11-C 100 ul
EUR 286

Purified Nipah virus Nucleoprotein control for Western Blotting

NIV21-C 100 ul
EUR 225

T-Pro Semi Dry Transfer Blotting buffer (10X)

JB08-H002 500ml/BT
EUR 144

Purified Lassa fever Nucleoprotein control for Western Blotting

LFNP15-C-10 100 ul
EUR 286

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow)

SA2020 1 kit(1200 cm2)
EUR 141

Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow)

SA2021 1 kit(1200 cm2)
EUR 141

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow)

SA2022 1 kit(1200 cm2)
EUR 141

Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow)

SA2023 1 kit(1200 cm2)
EUR 141

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue)

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EUR 141

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue)

SA2025 1 kit(1200 cm2)
EUR 141

GloBrite chemiluminescence reagent kit for western blotting 200 mL

GLB1 200 mL
EUR 145

GloBrite chemiluminescence reagent kit for western blotting 500 mL

GLB2 500 mL
EUR 234

Femto ECL substrate for ELISA/Western Blotting (2 Components)

80210 100 ml
EUR 347

Mayaro virus (MAYV) Capsid Protein control for Western Blotting

MAYV31-C 100 ul
EUR 286

Mayaro virus (MAYV) nsP1 protein control for Western Blotting

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Mayaro virus (MAYV) 6K Protein control for Western Blotting

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Mayaro virus (MAYV) E3 protein control for Western Blotting

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DiscoveryProbe? DNA Damage/DNA Repair Library

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Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco/Rbcl) Loading Control for Western Blotting

RBCL15-C 100 ul
EUR 286

pBR322 DNA

MSD14 50ug
EUR 152.66

pUC18 DNA

G40-300 50µg
EUR 56

pUC18 DNA

G40-305 5x50µg
EUR 147

DNA-fectamine

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EUR 278.81

DNA-Fect

DF37100-1 1 ml
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DNA-Fect293

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T7 DNA

310-005 200µg
EUR 82

T7 DNA

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abx023805-02mg 0.2 mg
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DNA Polymerase

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EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

DNA antibody

10R-D114b 100 ug
EUR 560
Description: Mouse monoclonal DNA antibody

DNA pol

ABF9055 100 ug
EUR 438

DNA antibody

10-D20A 500 ug
EUR 493
Description: Mouse monoclonal DNA antibody

DNA antibody

10C-CR6034M1 100 ug
EUR 241
Description: Mouse monoclonal DNA antibody

DNA Ligase I (DNA Ligase I) Antibody

abx232431-100ug 100 ug
EUR 481

Mouse Anti-DNA Antibody (DNA) ELISA Kit

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Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

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Western Blotting Boxes, 6-Compartment, 1 3/8 Height ( 32x100 mm Each component)

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DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

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Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-YP889065HU
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  • EUR 1442.00
  • EUR 382.00
  • 100ug
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

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1-CSB-BP889065HU
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  • EUR 1135.00
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  • EUR 470.00
  • 100ug
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-EP889065HU
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free

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Recombinant human DNA-directed DNA/RNA polymerase mu

P2815 100ug Ask for price
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu

DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

L1007-5 5 mg/well
EUR 5977
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Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

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Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated

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Taq DNA Polymerase

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pUC 18 DNA

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DNA Concentrator Kit

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Description: Ask the seller for details

Calf thymus DNA

HY-109517 100mg
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DNA Sample Diluent

D006-100 100 ml
EUR 373
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-1000 1000 ml
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

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DNA Precipitation Buffer

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Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Human DNA Concentrate

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EUR 506
Description: Human DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

E.coli DNA Concentrate

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EUR 542
Description: E.coli DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

CHO DNA Concentrate

D552 1 ml
EUR 542
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

CHO DNA Concentrate

D556 120 ul
EUR 469
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

DNA polymerase ? (Rat)

10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

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10-102 100ug
EUR 923
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DNA Pure Kit

FYG601-100P 100 Preps Ask for price

DNA Pure Kit

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BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

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IMMOLASE DNA Polymerase

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MyTaq DNA Polymerase

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Ranger DNA Polymerase

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Human Genomic DNA

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100bp DNA Marker

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EUR 165

100bp DNA Marker

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1Kb DNA Marker

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EUR 300

Human Genomic DNA

X11000 0.2 ml Ask for price
Description: reagents widely cited

DNA-PK Antibody

33358-100ul 100ul
EUR 252

DNA-PK Antibody

33358-50ul 50ul
EUR 187

Taq DNA Polymerase

BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

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EUR 145
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

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EUR 239
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

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EUR 522
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

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EUR 123
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 196
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

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EUR 413
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

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EUR 145
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

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EUR 239
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

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EUR 522
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

ClearSight DNA Stain

BH40501 1ml
EUR 103
Description: For DNA staining and vizualization.

Taq DNA polymerase

BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 120
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 190
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

100bp DNA ladder

9K-004-0002 0.5ml
EUR 217.48

DNA ligase Antibody

20-abx110554
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

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