Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.

Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.

Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.
Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.

Blotting roller

2322479 4unit
EUR 267

ECL Western Blotting Substrate

AR1170 200mL(sufficient reagents for 2000 cm2 of membrane)
EUR 149

ECL Plus Western Blotting Substrate

AR1196 50mL(sufficient reagents for 500 cm2 of membrane)
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ECL Western Blotting Substrate Kit

42R-1001 500 ml
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ECL Western Blotting Substrate Kit

K2187-50 50 assays
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ECL Western Blotting Substrate Kit

K2187-500 500 assays
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ECL Western Blotting Substrate Kit

K820-50
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ECL Western Blotting Substrate Kit

K820-500
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WESTSAVE Up (Western Blotting Substrate)

LF-QC0101 1 kit
EUR 151
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Purified Nipah virus Glycoprotein control for Western Blotting

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GloBrite chemiluminescence reagent kit for western blotting 200 mL

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80210 100 ml
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Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow)

SA2020 1 kit(1200 cm2)
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SA2021 1 kit(1200 cm2)
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DiscoveryProbe? DNA Damage/DNA Repair Library

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310-005 200µg
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310-025 5x200 µg
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Human DNA-directed DNA/RNA polymerase mu (POLM)

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  • MW: 58.8 kDa
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Human DNA-directed DNA/RNA polymerase mu (POLM)

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  • MW: 58.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Maximo DFS-Plus Taq DNA Polymerase DNA-free

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DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

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Recombinant human DNA-directed DNA/RNA polymerase mu

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  • Uniprot ID: Q9NP87
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
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DNA PK Antibody

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Pfu DNA Polymerase

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Taq DNA polymerase

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Taq DNA polymerase

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BT10202 1000U
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

DNA-PK Antibody

AF0550 200ul
EUR 304
Description: DNA-PK Antibody detects endogenous levels of DNA-PK.

Calf thymus DNA

HY-109517 100mg
EUR 1910

DNA Pure Kit

FYG601-100P 100 Preps Ask for price

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DNA Loading Dye

G030 6X; 3 x 1.0 ml
EUR 79

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 87

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 158

DNA ligase Antibody

20-abx110554
  • EUR 411.00
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Sapphire Baculovirus DNA

ABP-BVD-10001 15 µg (10 rxns) Ask for price
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DNA PK Antibody

abx149863-100ug 100 ug
EUR 439
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2K DNA Marker

20-abx098045
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20-abx098048
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15K DNA Marker

20-abx098049
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1Kb DNA Marker

20-abx098050
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100bp DNA Marker

20-abx098052
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Genomic DNA Kit

20-abx098076
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T4 DNA Ligase

abx098136-10kU 10 kU
EUR 321
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T4 DNA Ligase

abx098136-20Ku 20 Ku
EUR 398
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DNA Loading Buffer

abx098143-5ml 5 ml
EUR 175
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Circulating DNA Kit

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EUR 398
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Intercalated DNA Antibody

abx020668-02mg 0.2 mg
EUR 523
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DNA PK Antibody

ABF5340 100 ug
EUR 438

DNA- PK Antibody

ABF0550 100 ug
EUR 438

Circulating DNA Kit

abx298027-50rxns 50 rxns
EUR 398
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DNA PKcs antibody

70R-50261 100 ul
EUR 244
Description: Purified Polyclonal DNA PKcs antibody

Taq DNA Polymerase

9001-2500
EUR 512

Taq DNA Polymerase

9001-500
EUR 191

PFU DNA Polymerase

9003-2500
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PFU DNA Polymerase

9003-500
EUR 180

Laq? DNA Polymerase

9004-2500
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