Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.

Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.

Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.
Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.

ECL Plus Western Blotting Substrate

AR1196 50mL(sufficient reagents for 500 cm2 of membrane)
EUR 101

Blotting Paper, 10X15cm, 100 Sheets

PP3322 1PK, 100UNIT
EUR 105.68

Blotting Paper, 15X15cm, 100 Sheets

PP3323 1PK, 100UNIT
EUR 117.04

Blotting Paper, 20X20cm, 100 Sheets

PP3324 1PK, 100UNIT
EUR 166.4

ECL Western Blotting Substrate Kit

K2187-50 50 assays
EUR 160

ECL Western Blotting Substrate Kit

K2187-500 500 assays
EUR 363

WESTSAVE Up (Western Blotting Substrate)

LF-QC0101 1 kit
EUR 151
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

ECL Western Blotting Substrate Kit

K820-50
EUR 153

ECL Western Blotting Substrate Kit

K820-500
EUR 338

ECL Western Blotting Substrate Kit

42R-1001 500 ml
EUR 426
Description: ECL Western Blotting Substrate Kit for use in the research laboratory

T-Pro Transfer Blotting buffer (10X)

JB08-H001 500ml/BT
EUR 135

WESTSAVE Up (Western Blotting Substrate, double pack)

LF-QC0102 1 kit(Double pack)
EUR 222
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

Western Blotting Filter Paper, 12.5 cm—12.5 cm

AR0172 100 sheets
EUR 70

Western Blotting Filter Paper, 9 cm— 7.5 cm

AR0173 100 sheets
EUR 65

Purified Nipah virus Glycoprotein control for Western Blotting

NIV11-C 100 ul
EUR 286

Purified Nipah virus Nucleoprotein control for Western Blotting

NIV21-C 100 ul
EUR 225

T-Pro Semi Dry Transfer Blotting buffer (10X)

JB08-H002 500ml/BT
EUR 144

Purified Lassa fever Nucleoprotein control for Western Blotting

LFNP15-C-10 100 ul
EUR 286

GloBrite chemiluminescence reagent kit for western blotting 200 mL

GLB1 200 mL
EUR 145

GloBrite chemiluminescence reagent kit for western blotting 500 mL

GLB2 500 mL
EUR 234

Femto ECL substrate for ELISA/Western Blotting (2 Components)

80210 100 ml
EUR 347

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow)

SA2020 1 kit(1200 cm2)
EUR 141

Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow)

SA2021 1 kit(1200 cm2)
EUR 141

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow)

SA2022 1 kit(1200 cm2)
EUR 141

Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow)

SA2023 1 kit(1200 cm2)
EUR 141

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue)

SA2024 1 kit(1200 cm2)
EUR 141

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue)

SA2025 1 kit(1200 cm2)
EUR 141

Mayaro virus (MAYV) Capsid Protein control for Western Blotting

MAYV31-C 100 ul
EUR 286

Mayaro virus (MAYV) nsP1 protein control for Western Blotting

MAYV41-C 100 ul
EUR 286

Mayaro virus (MAYV) 6K Protein control for Western Blotting

MAYV51-C 100 ug
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Mayaro virus (MAYV) E3 protein control for Western Blotting

MAYV61-C 100 ul
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DiscoveryProbe? DNA Damage/DNA Repair Library

L1033-.1 100 uL/well(10 mM solution)
EUR 3889

DiscoveryProbe? DNA Damage/DNA Repair Library

L1033-.25 250 uL/well(10 mM solution)
EUR 6905

DiscoveryProbe? DNA Damage/DNA Repair Library

L1033-5 5 mg/well
EUR 9005

pUC18 DNA

G40-300 50µg
EUR 56

pUC18 DNA

G40-305 5x50µg
EUR 147

pBR322 DNA

MSD14 50ug
EUR 152.66

DNA-fectamine

DNAF001 1ml
EUR 278.81

T7 DNA

310-005 200µg
EUR 82

T7 DNA

310-025 5x200 µg
EUR 225

DNA Antibody

abx023805-02mg 0.2 mg
EUR 648

DNA-Fect

DF37100-1 1 ml
EUR 349

DNA-Fect293

DF37293-1 1 ml
EUR 291

DNA pol

ABF9055 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA antibody

10-D20A 500 ug
EUR 493
Description: Mouse monoclonal DNA antibody

DNA antibody

10C-CR6034M1 100 ug
EUR 241
Description: Mouse monoclonal DNA antibody

DNA antibody

10R-D114b 100 ug
EUR 560
Description: Mouse monoclonal DNA antibody

Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco/Rbcl) Loading Control for Western Blotting

RBCL15-C 100 ul
EUR 286

Mouse Anti-DNA Antibody (DNA) ELISA Kit

abx257816-96tests 96 tests
EUR 746

DNA Ligase I (DNA Ligase I) Antibody

abx232431-100ug 100 ug
EUR 481

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1525

Western Blotting Boxes, 6-Compartment, 1 3/8 Height ( 32x100 mm Each component)

LC7012-004 4/Pk
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Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 120

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 367

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-EP889065HU
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  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
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  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-BP889065HU
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  • EUR 332.00
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  • EUR 1135.00
  • EUR 1579.00
  • EUR 470.00
  • 100ug
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  • 200ug
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-YP889065HU
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  • EUR 900.00
  • EUR 1442.00
  • EUR 382.00
  • 100ug
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

Recombinant human DNA-directed DNA/RNA polymerase mu

P2815 100ug Ask for price
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu

DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

L1007-5 5 mg/well
EUR 5977
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc.

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated

AD-155-B Custom Ask for price

Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled

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DNA PK Antibody

abx149863-100ug 100 ug
EUR 439

DNA Sample Diluent

D006-100 100 ml
EUR 373
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-1000 1000 ml
EUR 1167
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DNA Sample Diluent

D006-500 500 ml
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Precipitation Buffer

D106 55 ml
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Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Human DNA Concentrate

D162 1 ml
EUR 506
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E.coli DNA Concentrate

D412 1 ml
EUR 542
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CHO DNA Concentrate

D552 1 ml
EUR 542
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CHO DNA Concentrate

D556 120 ul
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Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

EZ-DNA Reagents

BS8202 100preps
EUR 88.06

Calf thymus DNA

HY-109517 100mg
EUR 1910

DNA Concentrator Kit

P-1006 100 Samples
EUR 253
Description: Ask the seller for details

pUC 18 DNA

MSD15 50ug
EUR 122.21

pUC 19 DNA

MSD16 50ug
EUR 113.51

pUC 57 DNA

MSD17 50ug
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Phage λ-DNA

301-025 500 µg
EUR 102

Phage λ-DNA

301-100 5x500µg
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T4 DNA ligase

402-002 400 WEISS UNITS
EUR 63

T4 DNA ligase

402-010 2000 WEISS UNITS
EUR 154

DNA ligase Antibody

20-abx110554
  • EUR 411.00
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  • EUR 300.00
  • 100 ug
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100bp DNA ladder

9K-004-0002 0.5ml
EUR 217.48

2K DNA Marker

20-abx098045
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  • 2.5 ml
  • 500 ul

5K DNA Marker

20-abx098048
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  • 2.5 ml
  • 500 ul

15K DNA Marker

20-abx098049
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  • 2.5 ml
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1Kb DNA Marker

20-abx098050
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  • 2.5 ml
  • 500 ul

100bp DNA Marker

20-abx098052
  • EUR 272.00
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  • 2.5 ml
  • 500 ul

Genomic DNA Kit

20-abx098076
  • EUR 439.00
  • EUR 244.00
  • 200 rxns
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