Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.

Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.

Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.

CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.

ECL Western Blotting Substrate
KF005	Affbiotech	100ml	EUR 168

3MM CHR Blotting Paper 4x 5.25
F30306189	Scientific Laboratory Supplies	100PK	EUR 26.4

Nonfat Dry Milk Blotting Grade
20-241	Genesee Scientific	500g/Unit	EUR 272

Gel Blotting Paper 20X20 Gb005
426981	Scientific Laboratory Supplies	PK25	EUR 115.2

3MM CHR Blotting Paper 12x14CM
F30306132	Scientific Laboratory Supplies	100PK	EUR 34.8

ECL Prime Western Blotting Det
RPN2232	Scientific Laboratory Supplies	EACH	EUR 307.2

Blotting Paper, 10X15cm, 100 Sheets
PP3322	Bio Basic	1PK, 100UNIT	EUR 126.82

Blotting Paper, 15X15cm, 100 Sheets
PP3323	Bio Basic	1PK, 100UNIT	EUR 140.45

Blotting Paper, 20X20cm, 100 Sheets
PP3324	Bio Basic	1PK, 100UNIT	EUR 199.68

ECL PRIME WESTERN BLOTTING SYSTEM
GERPN2232	Scientific Laboratory Supplies	EACH	EUR 292.45

Slide blotting paper TSC 89X140mm
TS10020	Scientific Laboratory Supplies	PK1000	EUR 78

ECL Western Blotting Substrate Kit
42R-1001	Fitzgerald	500 ml	EUR 511.2
Description: ECL Western Blotting Substrate Kit for use in the research laboratory

ECL Western Blotting Substrate Kit
K820-50	Biovision	each	EUR 183.6

ECL Western Blotting Substrate Kit
K820-500	Biovision	each	EUR 405.6

ECL Western Blotting Substrate Kit
K2187-50	ApexBio	50 assays	EUR 192

ECL Western Blotting Substrate Kit
K2187-500	ApexBio	500 assays	EUR 435.6

ECL Plus Western Blotting Substrate
AR1196	BosterBio	50mL(sufficient reagents for 500 cm2 of membrane)	EUR 121.2

Whatman GB005 Blotting Paper 58x58cm
MOL3119	Scientific Laboratory Supplies	PK25	EUR 468

T-Pro Transfer Blotting buffer (10X)
JB08-H001	T-Pro Biotechnology	500ml/BT	EUR 162

WESTSAVE Up (Western Blotting Substrate)
LF-QC0101	Abfrontier	1 kit	EUR 181.2
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

WHATMAN GEL BLOTTING PAPER GRADE GB003
WHA10426892	Scientific Laboratory Supplies	PK50	EUR 481.2

OmniPAGE Blot Maxi blotting system 20x10cm
SB20	Scientific Laboratory Supplies	EACH	EUR 1764

WHATMAN GEL BLOTTING PAPERS GRADE GB005
WHA10426981	Scientific Laboratory Supplies	PK25	EUR 165.6

WHATMAN GEL BLOTTING PAPERS GRADE GB005
WHA10426994	Scientific Laboratory Supplies	PK25	EUR 582

Whatman GB003 70x100mm Gel Blotting Paper
10427806	Scientific Laboratory Supplies	PK100	EUR 22.8

Whatman GB003 100x100mm Gel Blotting Paper
MOL3107	Scientific Laboratory Supplies	PK50	EUR 33.6

Whatman GB003 300x600mm Gel Blotting Paper
MOL3117	Scientific Laboratory Supplies	PK25	EUR 116.4

Whatman GB004 150x150mm Gel Blotting Paper
MOL3118	Scientific Laboratory Supplies	PK25	EUR 93.6

WHATMAN NYTRAN N NYLON BLOTTING MEMBRANE
WHA10416094	Scientific Laboratory Supplies	EACH	EUR 524.4

WHATMAN NYTRAN N NYLON BLOTTING MEMBRANE
WHA10416196	Scientific Laboratory Supplies	EACH	EUR 697.2

Cytiva BLOTTING PAPER 14 X 16 CM 25 EA
GETE761416	Scientific Laboratory Supplies	PK25	EUR 62.7

Protran BA85 Blotting Membrane Circles 25MM
F10402578	Scientific Laboratory Supplies	1000PK	EUR 886.8

Western Blotting Filter Paper, 12.5 cm—12.5 cm
AR0172	BosterBio	100 sheets	EUR 84

Western Blotting Filter Paper, 9 cm— 7.5 cm
AR0173	BosterBio	100 sheets	EUR 78

Amersham ECL DualVue Western Blotting Markers
RPN810	Scientific Laboratory Supplies	EACH	EUR 265.2

Blotting Paper (Vitrobot Compatible), Pack of 100
M-CEM-VBBP100	MiTeGen	1 each	EUR 43
Description: Blotting Paper (Vitrobot Compatible), Pack of 100

T-Pro Semi Dry Transfer Blotting buffer (10X)
JB08-H002	T-Pro Biotechnology	500ml/BT	EUR 172.8

Whatman Potran BA 83 .2um 82mm Blotting Membrane
MOL3010	Scientific Laboratory Supplies	PK50	EUR 264

Whatman Potran BA 85 .45um 82mm Blotting Membrane
MOL3000	Scientific Laboratory Supplies	PK50	EUR 264

WESTSAVE Up (Western Blotting Substrate, double pack)
LF-QC0102	Abfrontier	1 kit(Double pack)	EUR 266.4
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

GloBrite chemiluminescence reagent kit for western blotting 200 mL
GLB1	Detroit R&D	200 mL	EUR 174

GloBrite chemiluminescence reagent kit for western blotting 500 mL
GLB2	Detroit R&D	500 mL	EUR 280.8

Western Blotting Boxes,15.2 x 10.2 x 5.1 cm (6 x 4 x 2in)
LC7021-005	GenDepot	5/Pk	EUR 102

Mayaro virus (MAYV) 6K Protein control for Western Blotting
MAYV51-C	Alpha Diagnostics	100 ug	EUR 343.2

Mayaro virus (MAYV) E3 protein control for Western Blotting
MAYV61-C	Alpha Diagnostics	100 ul	EUR 343.2

Western Blotting Boxes, 15.2 x 10.2 x 3.2 cm (6 x 4 x 1 1/4in)
LC7020-005	GenDepot	5/Pk	EUR 100.8

Mayaro virus (MAYV) nsP1 protein control for Western Blotting
MAYV41-C	Alpha Diagnostics	100 ul	EUR 343.2

Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow)
SA2023	BosterBio	1 kit(1200 cm2)	EUR 169.2

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue)
SA2024	BosterBio	1 kit(1200 cm2)	EUR 169.2

Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow)
SA2021	BosterBio	1 kit(1200 cm2)	EUR 169.2

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue)
SA2025	BosterBio	1 kit(1200 cm2)	EUR 169.2

Purified Nipah virus Glycoprotein control for Western Blotting
NIV11-C	Alpha Diagnostics	100 ul	EUR 343.2

Mayaro virus (MAYV) Capsid Protein control for Western Blotting
MAYV31-C	Alpha Diagnostics	100 ul	EUR 343.2

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow)
SA2020	BosterBio	1 kit(1200 cm2)	EUR 169.2

Western Blotting Boxes, 7.3 x 5.1 x 3.2 cm (2 7/8 x 2 x 1 1/4in)
LC7014-005	GenDepot	5/Pk	EUR 84

Western Blotting Boxes, 8.9 x 6.5 x 2.5 cm (3 1/2 x 2 9/16 x 1in)
LC7015-005	GenDepot	5/Pk	EUR 86.4

Purified Nipah virus Nucleoprotein control for Western Blotting
NIV21-C	Alpha Diagnostics	100 ul	EUR 270

Purified Lassa fever Nucleoprotein control for Western Blotting
LFNP15-C-10	Alpha Diagnostics	100 ul	EUR 343.2

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow)
SA2022	BosterBio	1 kit(1200 cm2)	EUR 169.2

Western Blotting Boxes, 7.3 x 3 x 1.9 cm (2 7/8 x 1 3/16 x 3/4in)
LC7013-005	GenDepot	5/Pk	EUR 84

Western Blotting Boxes, 9.5 x 3 x 1.6 cm (3 3/4 x 1 3/16 x 5/8in)
LC7016-005	GenDepot	5/Pk	EUR 86.4

Western Blotting Boxes,11.7 x 8.9 x 2.8 cm (4 5/8 x 3 1/2 x 1 1/8)
LC7018-005	GenDepot	5/Pk	EUR 92.4

Western Blotting Boxes, 11.6 x 11.6 x 3.2 cm (4 9/16 x 4 9/16 x 1 1/4)
LC7019-005	GenDepot	5/Pk	EUR 96

Western Blotting Boxes,11.1x 5.2 x 4.5 cm (4 3/8 x 2 1/16 x 1 25/328in)
LC7017-005	GenDepot	5/Pk	EUR 88.8

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane
RPN2106	Scientific Laboratory Supplies	EACH	EUR 374.4

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane
RPN2109	Scientific Laboratory Supplies	EACH	EUR 188.4

Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane
RPN2134	Scientific Laboratory Supplies	1KIT	EUR 572.4

Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane
RPN2209	Scientific Laboratory Supplies	EACH	EUR 291.6

Amersham ECL Western Blotting Systemor detection for 1000 cm2 membrane
RPN2108	Scientific Laboratory Supplies	EACH	EUR 326.4

Amersham ECL Plex Western Blotting Combination Pack Cy3 Cy5 HybondLFP
RPN999	Scientific Laboratory Supplies	EACH	EUR 280.8

Amersham ECL Plex Western Blotting Combination Pack Cy3 Cy5 Hybond ECL
RPN998	Scientific Laboratory Supplies	1KIT	EUR 304.8

Western Blotting Boxes, 6-Compartment, 1 3/8 Height ( 32x100 mm Each component)
LC7012-004	GenDepot	4/Pk	EUR 116.4

Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco/Rbcl) Loading Control for Western Blotting
RBCL15-C	Alpha Diagnostics	100 ul	EUR 343.2

MultiBox for Western blotting, 4 compartments, 32 x 52 x 28mm each (11/4 x 2 5/16 x 11/8 in.), for various gels
B1203	MTC Bio	6/pack	EUR 115.08
Description: Western Blot Boxes

MultiBox for Western blotting, 6 compartments, 33 x 103 x 35mm each (4 x 11/4 x 13/8 in.), for Protean 3 (cut-up)
B1206	MTC Bio	4/pack	EUR 110.63
Description: Western Blot Boxes

MultiBox for Western blotting, 2 compartments, 85 x 85 x 30mm each (311/32 x 311/32 x 13/16 in.), for Novex Mini (8x8cm), Bio-Rad Mini (7x8cm) and others
B1212	MTC Bio	6/pack	EUR 120.76
Description: Western Blot Boxes

MultiBox for Western blotting, 4 compartments, 38 x 71 x 28mm each (11/2 x 2 13/16 x 11/8 in.), for various gels including half-mini protein gels
B1204	MTC Bio	6/pack	EUR 119.14
Description: Western Blot Boxes

mini blotter 10x10 cm
EVS1100-BLOT	Consort	ea	EUR 818.4

maxi blotter 20x20 cm
EVS1300-BLOT	Consort	ea	EUR 1335.6

mini blotter 10x10 cm
EVS3100-BLOT	Consort	ea	EUR 1183.2

maxi blotter 20x20 cm
EVS3300-BLOT	Consort	ea	EUR 1639.2

mini wide blotter 20x10 cm
EVS1200-BLOT	Consort	ea	EUR 950.4

semi dry blotter mini 10x10 cm
ESDB1100	Consort	ea	EUR 625.2

semi dry blotter midi 20x20 cm
ESDB1200	Consort	ea	EUR 1335.6

semi dry blotter mini 10x10 cm
ESDB3100	Consort	ea	EUR 1030.8

semi dry blotter midi 20x20 cm
ESDB3200	Consort	ea	EUR 1486.8

Whatman 3mm Chr Blott 18x34cm
3030221	Scientific Laboratory Supplies	EACH	EUR 132

Mini semi dry blotter 10x10cm
SD10	Scientific Laboratory Supplies	EACH	EUR 621.47

Midi semi dry blotter 20x20cm
SD20	Scientific Laboratory Supplies	EACH	EUR 1331.71

DNA PK Blocking Peptide
AF5340-BP	Affbiotech	1mg	EUR 234

Blotto 1 buffer (5% milk, 0.05% tween in TBS buffer) for Western
80402-500	Alpha Diagnostics	500 ml	EUR 92.4

Blotter with non slip rubber mat platform(14x12"), 115V
BCM1621	Bio Basic	1 pcs, 1 UNIT	EUR 1301.72

Blotto for Western/ELISA (non-fat milk, makes ~25-L buffer @5%)
80401-250	Alpha Diagnostics	250 g	EUR 103.2

Blotto for Western/ELISA (non-fat milk, makes ~50-L buffer @5%)
80401-500	Alpha Diagnostics	500 g	EUR 132

Blood Genomic DNA Kit
abx098868-50rxns	Abbexa	50 rxns	EUR 326.4

DNA-PK Blocking Peptide
AF0550-BP	Affbiotech	1mg	EUR 234

DNA-PKcs Blocking Peptide
20-abx063136	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

Blood Advance? DNA Polymerase
M1153-100	Biovision	each	EUR 268.8

Blood Advance? DNA Polymerase
M1153-400	Biovision	each	EUR 568.8

Bloodirect DNA Polymerase (PCR)
G462	ABM	100 U (100 ul)	EUR 73.2

Bloodirect DNA Polymerase (PCR)
G463	ABM	400 U (400 ul)	EUR 104.4

ISOLATE II Blood DNA Kit
BIO-52062	Bioline	10 Preps	Ask for price

ISOLATE II Blood DNA Kit
BIO-52063	Bioline	50 Preps	Ask for price

Whole Blood DNA Isolation Kit
K528-100	Biovision	each	EUR 451.2

DNA Ligase 4 Blocking Peptide
20-abx062877	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

DNA Ligase 4 Blocking Peptide
20-abx061782	Abbexa	EUR 309.60 EUR 460.80	1 mg 5 mg

DNA Ligase 1 Blocking Peptide
20-abx061811	Abbexa	EUR 309.60 EUR 460.80	1 mg 5 mg

DNA Ligase 3 Blocking Peptide
20-abx061812	Abbexa	EUR 309.60 EUR 460.80	1 mg 5 mg

Blotto 2 buffer (1% milk, 1% BSA, 0.05% tween in TBS buffer for phosphoproteins) for Western
80403-500	Alpha Diagnostics	500 ml	EUR 92.4

DNA pol lambda Blocking Peptide
AF9055-BP	Affbiotech	1mg	EUR 234

DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-.1	ApexBio	100 uL/well(10 mM solution)	EUR 4666.8

DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-.25	ApexBio	250 uL/well(10 mM solution)	EUR 8286

DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-5	ApexBio	5 mg/well	EUR 10806

DNA Polymerase mu Blocking Peptide
20-abx061844	Abbexa	EUR 309.60 EUR 460.80	1 mg 5 mg

DNA-PKcs (pT2647) Blocking Peptide
20-abx162001	Abbexa	EUR 376.80 EUR 610.80	1 mg 5 mg

T7 DNA
310-005	GeneOn	200µg	EUR 98.4

T7 DNA
310-025	GeneOn	5x200 µg	EUR 270

DNA pol
ABF9055	Lifescience Market	100 ug	EUR 525.6

DNA Polymerase beta Blocking Peptide
20-abx063101	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

DNA Polymerase beta Blocking Peptide
AF0148-BP	Affbiotech	1mg	EUR 234

DNA Polymerase zeta Blocking Peptide
20-abx162018	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

pUC18 DNA
G40-300	GeneOn	50µg	EUR 67.2

pUC18 DNA
G40-305	GeneOn	5x50µg	EUR 176.4

DNA Polymerase alpha Blocking Peptide
AF5235-BP	Affbiotech	1mg	EUR 234

DNA Polymerase alpha Blocking Peptide
DF3082-BP	Affbiotech	1mg	EUR 234

DNA-Fect
DF37100-1	Neuromics	1 ml	EUR 418.8

pBR322 DNA
MSD14	Bio Basic	50ug	EUR 183.19

DNA Ligase I (DNA Ligase I) Antibody
abx232431-100ug	Abbexa	100 ug	EUR 577.2

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150	GeneOn	500 units	EUR 122.4

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152	GeneOn	5x500 units	EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154	GeneOn	20x500 units	EUR 1830

DNA antibody
10-D20A	Fitzgerald	500 ug	EUR 591.6
Description: Mouse monoclonal DNA antibody

DNA antibody
10C-CR6034M1	Fitzgerald	100 ug	EUR 289.2
Description: Mouse monoclonal DNA antibody

DNA antibody
10R-D114b	Fitzgerald	100 ug	EUR 672
Description: Mouse monoclonal DNA antibody

DNA Antibody
abx023805-02mg	Abbexa	0.2 mg	EUR 777.6

Activated DNA
80605	BPS Bioscience	500 µL	EUR 125
Description: Activated DNA for PARP assay, for use with BPS's assay kits for PARPs 1, 2, 3, and 6. PARP1 Chemiluminescent Assay Kit, #80551 ; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553; PARP6 Chemiluminescent Assay Kit, #80556; PARP2 Homogeneous Assay Kit, #80702

DNA-Fect293
DF37293-1	Neuromics	1 ml	EUR 349.2

DNA Polymerase
ABD3082	Lifescience Market	100 ug	EUR 525.6

DNA Polymerase
ABF5235	Lifescience Market	100 ug	EUR 525.6

Thymine DNA Glycosylase Blocking Peptide
20-abx162196	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

Blood and Serum DNA Isolation Kit
K5017100	Biochain	1 kit	EUR 355

DNA-fectamine
DNAF001	Bio Basic	1ml	EUR 334.57

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U	Alpha Diagnostics	Custom	Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B	Alpha Diagnostics	Custom	Ask for price