Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.

Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.

Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.

CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.

ECL Plus Western Blotting Substrate
AR1196	BosterBio	50mL(sufficient reagents for 500 cm2 of membrane)	EUR 101

Blotting Paper, 10X15cm, 100 Sheets
PP3322	Bio Basic	1PK, 100UNIT	EUR 105.68

Blotting Paper, 15X15cm, 100 Sheets
PP3323	Bio Basic	1PK, 100UNIT	EUR 117.04

Blotting Paper, 20X20cm, 100 Sheets
PP3324	Bio Basic	1PK, 100UNIT	EUR 166.4

ECL Western Blotting Substrate Kit
K2187-50	ApexBio	50 assays	EUR 160

ECL Western Blotting Substrate Kit
K2187-500	ApexBio	500 assays	EUR 363

WESTSAVE Up (Western Blotting Substrate)
LF-QC0101	Abfrontier	1 kit	EUR 151
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

ECL Western Blotting Substrate Kit
K820-50	Biovision		EUR 153

ECL Western Blotting Substrate Kit
K820-500	Biovision		EUR 338

ECL Western Blotting Substrate Kit
42R-1001	Fitzgerald	500 ml	EUR 426
Description: ECL Western Blotting Substrate Kit for use in the research laboratory

T-Pro Transfer Blotting buffer (10X)
JB08-H001	T-Pro Biotechnology	500ml/BT	EUR 135

WESTSAVE Up (Western Blotting Substrate, double pack)
LF-QC0102	Abfrontier	1 kit(Double pack)	EUR 222
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

Western Blotting Filter Paper, 12.5 cm—12.5 cm
AR0172	BosterBio	100 sheets	EUR 70

Western Blotting Filter Paper, 9 cm— 7.5 cm
AR0173	BosterBio	100 sheets	EUR 65

Purified Nipah virus Glycoprotein control for Western Blotting
NIV11-C	Alpha Diagnostics	100 ul	EUR 286

Purified Nipah virus Nucleoprotein control for Western Blotting
NIV21-C	Alpha Diagnostics	100 ul	EUR 225

T-Pro Semi Dry Transfer Blotting buffer (10X)
JB08-H002	T-Pro Biotechnology	500ml/BT	EUR 144

Purified Lassa fever Nucleoprotein control for Western Blotting
LFNP15-C-10	Alpha Diagnostics	100 ul	EUR 286

GloBrite chemiluminescence reagent kit for western blotting 200 mL
GLB1	Detroit R&D	200 mL	EUR 145

GloBrite chemiluminescence reagent kit for western blotting 500 mL
GLB2	Detroit R&D	500 mL	EUR 234

Femto ECL substrate for ELISA/Western Blotting (2 Components)
80210	Alpha Diagnostics	100 ml	EUR 347

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow)
SA2020	BosterBio	1 kit(1200 cm2)	EUR 141

Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow)
SA2021	BosterBio	1 kit(1200 cm2)	EUR 141

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow)
SA2022	BosterBio	1 kit(1200 cm2)	EUR 141

Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow)
SA2023	BosterBio	1 kit(1200 cm2)	EUR 141

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue)
SA2024	BosterBio	1 kit(1200 cm2)	EUR 141

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue)
SA2025	BosterBio	1 kit(1200 cm2)	EUR 141

Mayaro virus (MAYV) Capsid Protein control for Western Blotting
MAYV31-C	Alpha Diagnostics	100 ul	EUR 286

Mayaro virus (MAYV) nsP1 protein control for Western Blotting
MAYV41-C	Alpha Diagnostics	100 ul	EUR 286

Mayaro virus (MAYV) 6K Protein control for Western Blotting
MAYV51-C	Alpha Diagnostics	100 ug	EUR 286

Mayaro virus (MAYV) E3 protein control for Western Blotting
MAYV61-C	Alpha Diagnostics	100 ul	EUR 286

DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-.1	ApexBio	100 uL/well(10 mM solution)	EUR 3889

DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-.25	ApexBio	250 uL/well(10 mM solution)	EUR 6905

DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-5	ApexBio	5 mg/well	EUR 9005

pUC18 DNA
G40-300	GeneOn	50µg	EUR 56

pUC18 DNA
G40-305	GeneOn	5x50µg	EUR 147

pBR322 DNA
MSD14	Bio Basic	50ug	EUR 152.66

DNA-fectamine
DNAF001	Bio Basic	1ml	EUR 278.81

T7 DNA
310-005	GeneOn	200µg	EUR 82

T7 DNA
310-025	GeneOn	5x200 µg	EUR 225

DNA Antibody
abx023805-02mg	Abbexa	0.2 mg	EUR 648

DNA-Fect
DF37100-1	Neuromics	1 ml	EUR 349

DNA-Fect293
DF37293-1	Neuromics	1 ml	EUR 291

DNA pol
ABF9055	Lifescience Market	100 ug	EUR 438

DNA Polymerase
ABD3082	Lifescience Market	100 ug	EUR 438

DNA Polymerase
ABF5235	Lifescience Market	100 ug	EUR 438

DNA antibody
10-D20A	Fitzgerald	500 ug	EUR 493
Description: Mouse monoclonal DNA antibody

DNA antibody
10C-CR6034M1	Fitzgerald	100 ug	EUR 241
Description: Mouse monoclonal DNA antibody

DNA antibody
10R-D114b	Fitzgerald	100 ug	EUR 560
Description: Mouse monoclonal DNA antibody

Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco/Rbcl) Loading Control for Western Blotting
RBCL15-C	Alpha Diagnostics	100 ul	EUR 286

Mouse Anti-DNA Antibody (DNA) ELISA Kit
abx257816-96tests	Abbexa	96 tests	EUR 746

DNA Ligase I (DNA Ligase I) Antibody
abx232431-100ug	Abbexa	100 ug	EUR 481

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B	Alpha Diagnostics	Custom	Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U	Alpha Diagnostics	Custom	Ask for price

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150	GeneOn	500 units	EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152	GeneOn	5x500 units	EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154	GeneOn	20x500 units	EUR 1525

Western Blotting Boxes, 6-Compartment, 1 3/8 Height ( 32x100 mm Each component)
LC7012-004	GenDepot	4/Pk	EUR 97

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N140	GeneOn	500 units	EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N142	GeneOn	5x500 units	EUR 120

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N144	GeneOn	20x500 units	EUR 367

Human DNA-directed DNA/RNA polymerase mu (POLM)
1-CSB-EP889065HU	Cusabio	EUR 505.00 EUR 265.00 EUR 1827.00 EUR 766.00 EUR 1218.00 EUR 335.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Human DNA-directed DNA/RNA polymerase mu (POLM)
1-CSB-BP889065HU	Cusabio	EUR 840.00 EUR 332.00 EUR 2170.00 EUR 1135.00 EUR 1579.00 EUR 470.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Human DNA-directed DNA/RNA polymerase mu (POLM)
1-CSB-YP889065HU	Cusabio	EUR 586.00 EUR 299.00 EUR 2172.00 EUR 900.00 EUR 1442.00 EUR 382.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

Recombinant human DNA-directed DNA/RNA polymerase mu
P2815	FN Test	100ug	Ask for price
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu

DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel
L1007-5	ApexBio	5 mg/well	EUR 5977
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc.

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled
AD-146-F	Alpha Diagnostics	Custom	Ask for price

Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated
AD-155-B	Alpha Diagnostics	Custom	Ask for price

Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled
AD-155-U	Alpha Diagnostics	Custom	Ask for price

DNA PK Antibody
abx149863-100ug	Abbexa	100 ug	EUR 439

DNA Sample Diluent
D006-100	Cygnus Technologies	100 ml	EUR 373
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent
D006-1000	Cygnus Technologies	1000 ml	EUR 1167
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent
D006-500	Cygnus Technologies	500 ml	EUR 726
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Precipitation Buffer
D106	Cygnus Technologies	55 ml	EUR 175
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Human DNA Concentrate
D162	Cygnus Technologies	1 ml	EUR 506
Description: Human DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

E.coli DNA Concentrate
D412	Cygnus Technologies	1 ml	EUR 542
Description: E.coli DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

CHO DNA Concentrate
D552	Cygnus Technologies	1 ml	EUR 542
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

CHO DNA Concentrate
D556	Cygnus Technologies	120 ul	EUR 469
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

EZ-DNA Reagents
BS8202	Bio Basic	100preps	EUR 88.06

Calf thymus DNA
HY-109517	MedChemExpress	100mg	EUR 1910

DNA Concentrator Kit
P-1006	EpiGentek	100 Samples	EUR 253
Description: Ask the seller for details

pUC 18 DNA
MSD15	Bio Basic	50ug	EUR 122.21

pUC 19 DNA
MSD16	Bio Basic	50ug	EUR 113.51

pUC 57 DNA
MSD17	Bio Basic	50ug	EUR 113.51

Phage λ-DNA
301-025	GeneOn	500 µg	EUR 102

Phage λ-DNA
301-100	GeneOn	5x500µg	EUR 355

T4 DNA ligase
402-002	GeneOn	400 WEISS UNITS	EUR 63

T4 DNA ligase
402-010	GeneOn	2000 WEISS UNITS	EUR 154

DNA ligase Antibody
20-abx110554	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

100bp DNA ladder
9K-004-0002	Bio Basic	0.5ml	EUR 217.48

2K DNA Marker
20-abx098045	Abbexa	EUR 258.00 EUR 189.00	2.5 ml 500 ul

5K DNA Marker
20-abx098048	Abbexa	EUR 258.00 EUR 189.00	2.5 ml 500 ul

15K DNA Marker
20-abx098049	Abbexa	EUR 272.00 EUR 189.00	2.5 ml 500 ul

1Kb DNA Marker
20-abx098050	Abbexa	EUR 258.00 EUR 189.00	2.5 ml 500 ul

100bp DNA Marker
20-abx098052	Abbexa	EUR 272.00 EUR 189.00	2.5 ml 500 ul

Genomic DNA Kit
20-abx098076	Abbexa	EUR 439.00 EUR 244.00	200 rxns 50 rxns