Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.

Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.

Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.
Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.

ECL Western Blotting Substrate

AR1170 200mL(sufficient reagents for 2000 cm2 of membrane)
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ECL Western Blotting Substrate

KF005 100ml
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Ecl Plus Western Blotting

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ECL Western Blotting Substrate Kit

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ECL Western Blotting Substrate Kit

K820-50
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ECL Western Blotting Substrate Kit

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WESTSAVE Up (Western Blotting Substrate)

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ECL Western Blotting Substrate Kit

K2187-50 50 assays
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Blotting Paper, 10X15cm, 100 Sheets

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Western Blotting Filter Paper, 12.5 cm—12.5 cm

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Human DNA-directed DNA/RNA polymerase mu (POLM)

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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Human DNA-directed DNA/RNA polymerase mu (POLM)

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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

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Human DNA-directed DNA/RNA polymerase mu (POLM)

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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Recombinant human DNA-directed DNA/RNA polymerase mu

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DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

L1007-5 5 mg/well
EUR 7172.4
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc.

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
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DNA Sample Diluent

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DNA Sample Diluent

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DNA Sample Diluent

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DNA Precipitation Buffer

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100bp DNA Marker

D1100-050 50㎍
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EZ-DNA Reagents

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Taq DNA polymerase

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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BT10202 1000U
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

DNA PK Antibody

AF5340 200ul
EUR 420

DNA-PK Antibody

AF6631 100ul
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DNA-PK Antibody

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DNA PKcs antibody

70R-50261 100 ul
EUR 292.8
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DNA PKcs Antibody

48923-100ul 100ul
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DNA PKcs Antibody

48923-50ul 50ul
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DNA Assay Kit

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Uracil DNA Glycosylase

7-03778 2,000U Ask for price

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Taq DNA Polymerase

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Taq DNA Polymerase

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PFU DNA Polymerase

9003-2500
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9003-500
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Laq? DNA Polymerase

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9004-500
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T4 DNA ligase

402-002 400 WEISS UNITS
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T4 DNA ligase

402-010 2000 WEISS UNITS
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Z-DNA antibody

20-ZS20 250 ug
EUR 505.2
Description: Sheep polyclonal Z-DNA antibody

DNA PK antibody

20R-1466 100 ug
EUR 807.6
Description: Rabbit polyclonal DNA PK antibody

DNA PKcs antibody

20R-2017 50 ug
EUR 337.2
Description: Rabbit polyclonal DNA PKcs antibody

DNA PKcs antibody

20R-2346 50 ug
EUR 337.2
Description: Rabbit polyclonal DNA PKcs antibody

DNA-PK Antibody

3130-100
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DNA PKcs antibody

10R-10729 100 ug
EUR 457.2
Description: Mouse monoclonal DNA PKcs antibody

DNA-PK Antibody

33358-100ul 100ul
EUR 302.4

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