Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.

Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.

Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.

Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.
Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits.

CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.

Blotting Boxes, Large, Black

30-133 5 Boxes/Unit
EUR 42.44
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Electro Blotting Buffer 10X

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Blotting Paper Sheets - PK100

F3030-6188 PK100
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ECL Western Blotting Substrate

AR1170 200mL(sufficient reagents for 2000 cm2 of membrane)
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ECL Western Blotting Substrate

KF005 100ml
EUR 168

ECL Western Blotting Substrate

KF8005 100ml
EUR 140
Description: N/A

ECL Western Blotting Substrate

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Ecl Plus Western Blotting - PK3

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TMB Solution for Western Blotting

18186-24 200ML
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30-131 5 Boxes/Unit
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Description: 11.1x 5.2 x 4.7cm

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Description: 7.3 x 3 x 1.9cm

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42R-1001 500 ml
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Description: ECL Western Blotting Substrate Kit for use in the research laboratory

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WSHT-ZY5 Western Blotting System

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Description: 7.3 x 5.1 x 3.2cm

Gel Blotting Paper 20X20 Gb005 - PK25

426981 PK25
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3MM CHR Blotting Paper 12x14CM - 100PK

F30306132 100PK
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ECL Prime Western Blotting Det - EACH

RPN2232 EACH
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WESTSAVE Up (Western Blotting Substrate)

LF-QC0101 1 kit
EUR 181.2
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

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Description: 9 x 6.5 x 2.5cm

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30-139 5 Boxes/Unit
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Description: 9.0 x 6.5 x 2.5cm

TBST 20X, pH 7.5 for Western Blotting

40120065-1 500 mL
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TBST 20X, pH 7.5 for Western Blotting

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Prometheus® NC Blotting Membrane, 0.2um

84-875 1 Roll/Unit
EUR 319.74
Description: 300mm x 3m

ECL PRIME WESTERN BLOTTING SYSTEM - EACH

GERPN2232 EACH
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HiPer® Western Blotting Teaching Kit

HTI009-20PR 1 unit
EUR 274.64
Description: HiPer® Western Blotting Teaching Kit

HiPer® Western Blotting Teaching Kit

HTI009-5PR 1 unit
EUR 82.63
Description: HiPer® Western Blotting Teaching Kit

Western Blotting Antibody Dilution Buffer

E-IR-R121-100mL 100mL
EUR 40

Western Blotting Antibody Dilution Buffer

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Western Blotting Antibody Dilution Buffer

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Western Blotting Antibody Dilution Buffer

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84-876 1 Roll/Unit
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Description: 300mm x 3m

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ML210-25R 1 unit
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Description: HiPurA® Fast Western Blotting Buffers

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Bullet Blocking One for Western Blotting

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Bullet Blocking One for Western Blotting

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Bullet Blocking One for Western Blotting

13779-56 50ML
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AgileBlotter TM Automated Blotting Handler

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Prometheus® PVDF Blotting Membrane, 0.2um

84-895 1 Roll/Unit
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Description: 26.5cm x 3.3m

Slide blotting paper TSC 89X140mm - PK1000

TS10020 PK1000
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T-Pro HQ Transfer Blotting buffer (20X)

JB08-H003 500ml/BT
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Prometheus® PVDF Blotting Membrane, 0.45um

83-646R 1 Roll/Unit
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Description: 300mm x 3m

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30-142 10 Boxes/Unit
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Description: 8.6 x 8.6 x 2.8cm

Whatman GB005 Blotting Paper 58x58cm - PK25

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WHATMAN NYTRAN N NYLON BLOTTING MEMBRANE

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Super ECL Plus Western Blotting Substrate

EZWB01-SEP100 2x100mL
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Cytiva BLOTTING PAPER 14 X 16 CM 25 EA

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MBM023-20SH 1 unit
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Description: HiFiBloE™ Nylon Membrane for Blotting, 3

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Description: HiFiBloE™ Nylon Membrane for Blotting, 3

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MBM025-1RL 1 unit
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HiPurA® Fast Unik Western Blotting Buffe

ML211-25R 1 unit
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Description: HiPurA® Fast Unik Western Blotting Buffe

HiPurA® Fast Unik Western Blotting Buffe

ML211-5R 1 unit
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Western Blotting Filter Paper, 12.5 cm—12.5 cm

AR0172 100 sheets
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Western Blotting Filter Paper, 9 cm— 7.5 cm

AR0173 100 sheets
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WHATMAN GEL BLOTTING PAPER GRADE GB003 - PK50

WHA10426892 PK50
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OmniPAGE Blot Maxi blotting system 20x10cm - EACH

SB20 EACH
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WHATMAN GEL BLOTTING PAPERS GRADE GB005 - PK25

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ProSignal® ECL Blotting Film, 5 x 7 in.

30-507L 100 Sheets/Unit
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Description: Double Emulsion

ProSignal® ECL Blotting Film, 8 x 10 in.

30-810L 100 Sheets/Unit
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Description: Double Emulsion

Rapid Western Blotting Kit Rat-Wet Transfer

20750009-1 1 Kit
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Blotting Paper (Vitrobot Compatible), Pack of 100

M-CEM-VBBP100 1 each
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Description: Blotting Paper (Vitrobot Compatible), Pack of 100

Rapid Western Blotting Kit Goat-Wet Transfer

20750007-1 1 Kit
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Whatman GB003 100x100mm Gel Blotting Paper - PK50

MOL3107 PK50
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Whatman GB003 300x600mm Gel Blotting Paper - PK25

MOL3117 PK25
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Whatman GB004 150x150mm Gel Blotting Paper - PK25

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WHATMAN NYTRAN N NYLON BLOTTING MEMBRANE - EACH

WHA10416094 EACH
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T-Pro Semi Dry Transfer Blotting buffer (10X)

JB08-H002 500ml/BT
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Whatman GB003 70x100mm Gel Blotting Paper - PK100

10427806 PK100
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Protran BA85 Blotting Membrane Circles 25MM - 1000PK

F10402578 1000PK
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WESTSAVE Up (Western Blotting Substrate, double pack)

LF-QC0102 1 kit(Double pack)
EUR 266.4
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

Amersham ECL DualVue Western Blotting Markers - EACH

RPN810 EACH
EUR 298.35

Rapid Western Blotting Kit Rat-Semi Dry Transfer

20750008-1 1 Kit
EUR 181.45

Rapid Western Blotting Kit Goat-Semi Dry Transfer

20750006-1 1 Kit
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GloBrite chemiluminescence reagent kit for western blotting 200 mL

GLB1 200 mL
EUR 137

GloBrite chemiluminescence reagent kit for western blotting 500 mL

GLB2 500 mL
EUR 297

Rapid Western Blotting Kit Mouse-Semi Dry Transfer

20750000-1 1 Kit
EUR 181.45

Whatman Potran BA 83 .2um 82mm Blotting Membrane - PK50

MOL3010 PK50
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Whatman Potran BA 85 .45um 82mm Blotting Membrane - PK50

MOL3000 PK50
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Western Blotting Boxes,15.2 x 10.2 x 5.1 cm (6 x 4 x 2in)

LC7021-005 5/Pk
EUR 102

Mayaro virus (MAYV) 6K Protein control for Western Blotting

MAYV51-C 100 ug
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Mayaro virus (MAYV) E3 protein control for Western Blotting

MAYV61-C 100 ul
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Signal Enhancer HIKARI for Western Blotting and ELISA (50ml)

02267-41 1SET
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Signal Enhancer HIKARI for Western Blotting and ELISA (250ml)

02270-81 1SET
EUR 210

Mayaro virus (MAYV) nsP1 protein control for Western Blotting

MAYV41-C 100 ul
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Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow)

SA2023 1 kit(1200 cm2)
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Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue)

SA2024 1 kit(1200 cm2)
EUR 169.2

Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow)

SA2021 1 kit(1200 cm2)
EUR 169.2

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue)

SA2025 1 kit(1200 cm2)
EUR 169.2

Western Blotting Boxes, 15.2 x 10.2 x 3.2 cm (6 x 4 x 1 1/4in)

LC7020-005 5/Pk
EUR 100.8

Purified Nipah virus Glycoprotein control for Western Blotting

NIV11-C 100 ul
EUR 343.2

Mayaro virus (MAYV) Capsid Protein control for Western Blotting

MAYV31-C 100 ul
EUR 343.2

Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow)

SA2020 1 kit(1200 cm2)
EUR 169.2

Purified Lassa fever Nucleoprotein control for Western Blotting

LFNP15-C-10 100 ul
EUR 343.2

Purified Nipah virus Nucleoprotein control for Western Blotting

NIV21-C 100 ul
EUR 270

Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow)

SA2022 1 kit(1200 cm2)
EUR 169.2

Western Blotting Boxes, 7.3 x 5.1 x 3.2 cm (2 7/8 x 2 x 1 1/4in)

LC7014-005 5/Pk
EUR 84

Western Blotting Boxes, 8.9 x 6.5 x 2.5 cm (3 1/2 x 2 9/16 x 1in)

LC7015-005 5/Pk
EUR 86.4

Signal Enhancer HIKARI for Western Blotting and ELISA Solution A

02272-74 250ML
EUR 119

Signal Enhancer HIKARI for Western Blotting and ELISA Solution B

02297-64 250ML
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Western Blotting Boxes, 7.3 x 3 x 1.9 cm (2 7/8 x 1 3/16 x 3/4in)

LC7013-005 5/Pk
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Western Blotting Boxes, 9.5 x 3 x 1.6 cm (3 3/4 x 1 3/16 x 5/8in)

LC7016-005 5/Pk
EUR 86.4

Western Blotting Boxes,11.7 x 8.9 x 2.8 cm (4 5/8 x 3 1/2 x 1 1/8)

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Western Blotting Boxes, 11.6 x 11.6 x 3.2 cm (4 9/16 x 4 9/16 x 1 1/4)

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EUR 96

Western Blotting Boxes,11.1x 5.2 x 4.5 cm (4 3/8 x 2 1/16 x 1 25/328in)

LC7017-005 5/Pk
EUR 88.8

Neurexin 1 (NRXN1) (blotting) mouse monoclonal antibody, clone N170A/26

73-217 5 ml Ask for price

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane - EACH

RPN2106 EACH
EUR 421.2

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane - EACH

RPN2109 EACH
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Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane - 1KIT

RPN2134 1KIT
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Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane - EACH

RPN2209 EACH
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Amersham ECL Western Blotting Systemor detection for 1000 cm2 membrane - EACH

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