BACKGROUNDSand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop mobile and humoral responses in opposition to sand fly saliva. A optimistic correlation has been noticed between the quantity of bites and antibody ranges indicating that anti-saliva antibody response can be utilized as marker of publicity to sand flies.
Little is thought about kinetics of antibodies in opposition to Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work centered on the examine of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that had been experimentally uncovered to the bites of uninfected sand flies.RESULTSAnti-saliva antibodies had been evaluated by ELISA and Western blot. In addition, antibody ranges in opposition to two P. perniciosus rSP, apyrase rSP01B and D7 associated protein rSP04 had been decided in mice sera.
Anti-saliva antibody ranges elevated alongside the immunizations and correlated with the quantity of sand fly bites. Anti-SGH antibody ranges had been detected in sera of mice 5 weeks after publicity, and endured for at the very least three months. Anti-apyrase rSP01B antibodies adopted comparable kinetic responses than anti-SGH antibodies whereas rSP04 confirmed a delayed response and exhibited a higher variability amongst sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of publicity and IgG antibodies endured at excessive ranges, even 7 months post-exposure.
Kinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbits. CONCLUSIONSOur outcomes contributed to extend the data on the kind of immune response P. perniciosus saliva and particular person proteins elicited highlighting the use of rSP01B as an epidemiological marker of publicity. Anti-saliva kinetics in sera of experimentally bitten rabbits had been studied for the primary time. Results with rabbit mannequin supplied helpful info for a greater understanding of the anti-saliva antibody ranges discovered in wild leporids in the human leishmaniasis focus in the Madrid area, Spain.
ECL Western Blotting Substrate
KF005
Affbiotech
100ml
EUR 140
Blotting Paper, 10X15cm, 100 Sheets
PP3322
Bio Basic
1PK, 100UNIT
EUR 105.68
Blotting Paper, 15X15cm, 100 Sheets
PP3323
Bio Basic
1PK, 100UNIT
EUR 117.04
Blotting Paper, 20X20cm, 100 Sheets
PP3324
Bio Basic
1PK, 100UNIT
EUR 166.4
ECL Plus Western Blotting Substrate
AR1196
BosterBio
50mL(sufficient reagents for 500 cm2 of membrane)
EUR 101
ECL Western Blotting Substrate Kit
K2187-50
ApexBio
50 assays
EUR 160
ECL Western Blotting Substrate Kit
K2187-500
ApexBio
500 assays
EUR 363
WESTSAVE Up (Western Blotting Substrate)
LF-QC0101
Abfrontier
1 kit
EUR 151
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent
substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.
ECL Western Blotting Substrate Kit
42R-1001
Fitzgerald
500 ml
EUR 426
Description: ECL Western Blotting Substrate Kit for use in the research laboratory
ECL Western Blotting Substrate Kit
K820-50
Biovision
EUR 153
ECL Western Blotting Substrate Kit
K820-500
Biovision
EUR 338
WESTSAVE Up (Western Blotting Substrate, double pack)
LF-QC0102
Abfrontier
1 kit(Double pack)
EUR 222
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent
substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.
Western Blotting Filter Paper, 12.5 cmā12.5 cm
AR0172
BosterBio
100 sheets
EUR 70
Western Blotting Filter Paper, 9 cmā 7.5 cm
AR0173
BosterBio
100 sheets
EUR 65
Purified Nipah virus Glycoprotein control for Western Blotting
NIV11-C
Alpha Diagnostics
100 ul
EUR 286
Purified Nipah virus Nucleoprotein control for Western Blotting
NIV21-C
Alpha Diagnostics
100 ul
EUR 225
Purified Lassa fever Nucleoprotein control for Western Blotting
LFNP15-C-10
Alpha Diagnostics
100 ul
EUR 286
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Yellow)
SA2020
BosterBio
1 kit(1200 cm2)
EUR 141
Western Blotting Goat IgG DAB Chromogenic Reagent Kit (Yellow)
SA2021
BosterBio
1 kit(1200 cm2)
EUR 141
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Yellow)
SA2022
BosterBio
1 kit(1200 cm2)
EUR 141
Western Blotting Rat IgG DAB Chromogenic Reagent Kit (Yellow)
SA2023
BosterBio
1 kit(1200 cm2)
EUR 141
Western Blotting Mouse IgG DAB Chromogenic Reagent Kit (Blue)
SA2024
BosterBio
1 kit(1200 cm2)
EUR 141
Western Blotting Rabbit IgG DAB Chromogenic Reagent Kit (Blue)
SA2025
BosterBio
1 kit(1200 cm2)
EUR 141
GloBrite chemiluminescence reagent kit for western blotting 200 mL
GLB1
Detroit R&D
200 mL
EUR 145
GloBrite chemiluminescence reagent kit for western blotting 500 mL
GLB2
Detroit R&D
500 mL
EUR 234
Femto ECL substrate for ELISA/Western Blotting (2 Components)
80210
Alpha Diagnostics
100 ml
EUR 347
Mayaro virus (MAYV) Capsid Protein control for Western Blotting
MAYV31-C
Alpha Diagnostics
100 ul
EUR 286
Mayaro virus (MAYV) nsP1 protein control for Western Blotting
MAYV41-C
Alpha Diagnostics
100 ul
EUR 286
Mayaro virus (MAYV) 6K Protein control for Western Blotting
MAYV51-C
Alpha Diagnostics
100 ug
EUR 286
Mayaro virus (MAYV) E3 protein control for Western Blotting
MAYV61-C
Alpha Diagnostics
100 ul
EUR 286
DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-.1
ApexBio
100 uL/well(10 mM solution)
EUR 3889
DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-.25
ApexBio
250 uL/well(10 mM solution)
EUR 6905
DiscoveryProbe? DNA Damage/DNA Repair Library
L1033-5
ApexBio
5 mg/well
EUR 9005
Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco/Rbcl) Loading Control for Western Blotting
RBCL15-C
Alpha Diagnostics
100 ul
EUR 286
pUC18 DNA
G40-300
GeneOn
50Āµg
EUR 56
pUC18 DNA
G40-305
GeneOn
5x50Āµg
EUR 147
DNA-fectamine
DNAF001
Bio Basic
1ml
EUR 278.81
T7 DNA
310-005
GeneOn
200Āµg
EUR 82
T7 DNA
310-025
GeneOn
5x200 Āµg
EUR 225
DNA Antibody
abx023805-02mg
Abbexa
0.2 mg
EUR 648
DNA antibody
10R-D114b
Fitzgerald
100 ug
EUR 560
Description: Mouse monoclonal DNA antibody
DNA antibody
10-D20A
Fitzgerald
500 ug
EUR 493
Description: Mouse monoclonal DNA antibody
DNA antibody
10C-CR6034M1
Fitzgerald
100 ug
EUR 241
Description: Mouse monoclonal DNA antibody
DNA Ligase I (DNA Ligase I) Antibody
abx232431-100ug
Abbexa
100 ug
EUR 481
Mouse Anti-DNA Antibody (DNA) ELISA Kit
abx257816-96tests
Abbexa
96 tests
EUR 746
Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B
Alpha Diagnostics
Custom
Ask for price
Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U
Alpha Diagnostics
Custom
Ask for price
Western Blotting Boxes, 6-Compartment, 1 3/8 Height ( 32x100 mm Each component)
LC7012-004
GenDepot
4/Pk
EUR 97
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150
GeneOn
500 units
EUR 102
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152
GeneOn
5x500 units
EUR 394
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154
GeneOn
20x500 units
EUR 1525
Human DNA-directed DNA/RNA polymerase mu (POLM)
1-CSB-YP889065HU
Cusabio
EUR 586.00
EUR 299.00
EUR 2172.00
EUR 900.00
EUR 1442.00
EUR 382.00
100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast
Human DNA-directed DNA/RNA polymerase mu (POLM)
1-CSB-BP889065HU
Cusabio
EUR 840.00
EUR 332.00
EUR 2170.00
EUR 1135.00
EUR 1579.00
EUR 470.00
100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus
Human DNA-directed DNA/RNA polymerase mu (POLM)
1-CSB-EP889065HU
Cusabio
EUR 505.00
EUR 265.00
EUR 1827.00
EUR 766.00
EUR 1218.00
EUR 335.00
100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N140
GeneOn
500 units
EUR 50
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N142
GeneOn
5x500 units
EUR 120
Maximo DFS-Plus Taq DNA Polymerase DNA-free
N144
GeneOn
20x500 units
EUR 367
Recombinant human DNA-directed DNA/RNA polymerase mu
P2815
FN Test
100ug
Ask for price
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu
DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel
L1007-5
ApexBio
5 mg/well
EUR 5977
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc.
Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled
AD-146-F
Alpha Diagnostics
Custom
Ask for price
Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated
AD-155-B
Alpha Diagnostics
Custom
Ask for price
Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled
AD-155-U
Alpha Diagnostics
Custom
Ask for price
Taq DNA Polymerase
BA00103
Cusabio
500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.
EZ-DNA Reagents
SK8201
Bio Basic
100preps
EUR 93.5
Pfu DNA Polymerase
S116
GeneOn
1x250 units
EUR 76
Pfu DNA Polymerase
S117
GeneOn
2x250 units
EUR 94
Pfu DNA Polymerase
S118
GeneOn
10x250 units
EUR 303
Bst DNA Polymerase
S600
GeneOn
2000 U
EUR 107
Green-DNA Dye
SB2040
Bio Basic
100UL, 100UL
EUR 67.4
DNA Concentrator Kit
P-1006
EpiGentek
100 Samples
EUR 253
Description: Ask the seller for details
DNA Sample Diluent
D006-100
Cygnus Technologies
100 ml
EUR 373
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.
DNA Sample Diluent
D006-1000
Cygnus Technologies
1000 ml
EUR 1167
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.
DNA Sample Diluent
D006-500
Cygnus Technologies
500 ml
EUR 726
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.
DNA Precipitation Buffer
D106
Cygnus Technologies
55 ml
EUR 175
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.
Human DNA Concentrate
D162
Cygnus Technologies
1 ml
EUR 506
Description: Human DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.
E.coli DNA Concentrate
D412
Cygnus Technologies
1 ml
EUR 542
Description: E.coli DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.
CHO DNA Concentrate
D552
Cygnus Technologies
1 ml
EUR 542
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.
CHO DNA Concentrate
D556
Cygnus Technologies
120 ul
EUR 469
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.
DNA polymerase ? (Rat)
10-101
Sceti
20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.
DNA polymerase ? (Rat)
10-102
Sceti
100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.
BIOTAQ DNA Polymerase
BIO-21040
Bioline
500 Units
Ask for price
IMMOLASE DNA Polymerase
BIO-21046
Bioline
250 Units
Ask for price
IMMOLASE DNA Polymerase
BIO-21047
Bioline
500 Units
Ask for price
IMMOLASE DNA Polymerase
BIO-21048
Bioline
5000 Units
Ask for price
ACCUZYME DNA Polymerase
BIO-21051
Bioline
250 Units
Ask for price
ACCUZYME DNA Polymerase
BIO-21052
Bioline
500 Units
Ask for price
BIOTAQ DNA Polymerase
BIO-21060
Bioline
2500 Units
Ask for price
MangoTaq DNA Polymerase
BIO-21078
Bioline
5000 Units
Ask for price
MangoTaq DNA Polymerase
BIO-21082
Bioline
2000 Units
Ask for price
MangoTaq DNA Polymerase
BIO-21083
Bioline
1000 Units
Ask for price
VELOCITY DNA Polymerase
BIO-21098
Bioline
250 Units
Ask for price
VELOCITY DNA Polymerase
BIO-21099
Bioline
500 Units
Ask for price
MyTaq DNA Polymerase
BIO-21105
Bioline
500 units
Ask for price
MyTaq DNA Polymerase
BIO-21105/S
Bioline
Sample
Ask for price
MyTaq DNA Polymerase
BIO-21106
Bioline
2500 units
Ask for price
MyTaq DNA Polymerase
BIO-21107
Bioline
5000 units
Ask for price
MyFi DNA Polymerase
BIO-21117
Bioline
250 Units
Ask for price
MyFi DNA Polymerase
BIO-21117/S
Bioline
Sample
Ask for price
MyFi DNA Polymerase
BIO-21118
Bioline
500 Units
Ask for price
MyFi DNA Polymerase
BIO-21119
Bioline
2500 Units
Ask for price
Ranger DNA Polymerase
BIO-21121
Bioline
250 Units
Ask for price
Ranger DNA Polymerase
BIO-21121/S
Bioline
Sample
Ask for price
Ranger DNA Polymerase
BIO-21122
Bioline
500 Units
Ask for price
Human Genomic DNA
BIO-35025
Bioline
500Āµl @ 200ng/Āµl
Ask for price
100bp DNA Marker
D1100-050
GenDepot
50ć
EUR 165
100bp DNA Marker
D1100-250
GenDepot
5X50ć
EUR 550
1Kb DNA Marker
D1101-050
GenDepot
50ć
EUR 131
1Kb DNA Marker
D1101-250
GenDepot
5X50ć
EUR 300
Human Genomic DNA
X11000
EpiGentek
0.2 ml
Ask for price
Description: reagents widely cited
DNA-PK Antibody
33358-100ul
SAB
100ul
EUR 252
DNA-PK Antibody
33358-50ul
SAB
50ul
EUR 187
Taq DNA Polymerase
BA00104
Bioatlas
1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00105
Bioatlas
2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.
HotTaq DNA Polymerase
BA00203
Bioatlas
500U
EUR 145
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq DNA Polymerase
BA00204
Bioatlas
1000U
EUR 239
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq DNA Polymerase
BA00205
Bioatlas
2500U
EUR 522
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
RedTaq DNA Polymerase
BA00303
Bioatlas
500U
EUR 123
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.
RedTaq DNA Polymerase
BA00304
Bioatlas
1000U
EUR 196
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.
RedTaq DNA Polymerase
BA00305
Bioatlas
2500U
EUR 413
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.
Pfu DNA Polymerase
BA00503
Bioatlas
500U
EUR 145
Description: High quality Pfu polymerase for different PCR variations and downstream applications.
Pfu DNA Polymerase
BA00504
Bioatlas
1000U
EUR 239
Description: High quality Pfu polymerase for different PCR variations and downstream applications.
Pfu DNA Polymerase
BA00505
Bioatlas
2500U
EUR 522
Description: High quality Pfu polymerase for different PCR variations and downstream applications.
ClearSight DNA Stain
BH40501
Bioatlas
1ml
EUR 103
Description: For DNA staining and vizualization.
Taq DNA polymerase
BT10101
Bioatlas
500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10102
Bioatlas
1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10103
Bioatlas
2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.
HotTaq DNA polymerase
BT10201
Bioatlas
500U
EUR 120
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
HotTaq DNA polymerase
BT10202
Bioatlas
1000U
EUR 190
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.
100bp DNA ladder
9K-004-0002
Bio Basic
0.5ml
EUR 217.48
DNA ligase Antibody
20-abx110554
Abbexa
EUR 411.00
EUR 1845.00
EUR 599.00
EUR 182.00
EUR 300.00
100 ug 1 mg 200 ug 20 ug 50 ug
